[1]梁燕,韩传明*,孙超,等.基于AFLP标记的薄壳山核桃种质资源遗传多样性分析[J].江苏林业科技,2022,49(01):1-7.[doi:10.3969/j.issn.1001-7380.2022.01.001]
　Liang Yan,Han Chuanming*,Sun Chao,et al.Genetic diversity analysis of pecan germplasm resources based on AFLP markers[J].Journal of Jiangsu Forestry Science &Technology,2022,49(01):1-7.[doi:10.3969/j.issn.1001-7380.2022.01.001]
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基于AFLP标记的薄壳山核桃种质资源遗传多样性分析()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第49卷
期数:
2022年01期
页码:
1-7
栏目:
试验研究
出版日期:
2022-03-05

文章信息/Info

Title:
Genetic diversity analysis of pecan germplasm resources based on AFLP markers
文章编号:
1001-7380（2022）01-0001-07
作者:
梁燕1; 韩传明1*; 孙超1; 王翠香1; 闵旭峰2; 王静1; 王清海1
1.山东省林业科学研究院，山东 济南 250014； 2.枣庄市山亭区自然资源局，山东 枣庄 277500
Author(s):
Liang Yan1; Han Chuanming1*;  Sun Chao1; Wang Cuixiang1; Min Xufeng2; Wang Jing1; Wang Qinghai1
1.Shandong Academy of Forestry, Jinan 250014,China； 2.Zaozhuang Shanting District Natural Resources Bureau, Zaozhuang 277500,China
关键词:
AFLP分子标记; 薄壳山核桃; 遗传多样性; 遗传相似系数; 山东
Keywords:
AFLP; Pecan; Genetic diversity; Genetic similarity; Shandong
分类号:
Q755S664.1S792.13
DOI:
10.3969/j.issn.1001-7380.2022.01.001
文献标志码:
A
摘要:
利用荧光AFLP分子标记技术，对引种到山东地区的30份薄壳山核桃品种（系）的遗传多样性和亲缘关系进行分析，旨在加深山东地区引进的薄壳山核桃品种资源遗传背景的认识，为以后山东地区薄壳山核桃的品种选育在分子层面提供理论基础。总体上，9对引物组合共扩增出1 425个条带，多态性条带1 381 个，平均多态性比例为96.9%，其中有4个引物组合的多态性比率达到100%。30个薄壳山核桃品种（系）利用9对引物组合所检测到的不同位点的等位基因数范围在1.652 8—1.888 9之间，平均为1.710 3；有效等位基因数范围在1.182 1—1.306 8之间，平均为1.240 3；基因多样度范围在0.120 1—0.190 9之间，平均为0.152 2；Shannon信息指数范围在0.200 0—0.304 8之间，平均为0.245 5。30个供试样品遗传相似系数范围为0.746 4—0.885 3，变异范围为小（0.138 9），表明30个品种遗传背景宽度较窄。在遗传相似系数0.796处，30个薄壳山核桃品种分为3个组，小糙皮系列聚为一类，与其他品种遗传相似系数整体较小，是很好的亲本，值得更深一步研究与利用。
Abstract:
To understand the genetic background of pecan varieties and provide basis for breeding at molecular level, the genetic diversity and genetic relationship of 30 pecan varieties (or lines) introduced to Shandong region, were analyzed by using the fluorescent markers and molecular technology. In total, 1 425 bands and 1 381 polymorphic bands ( a ratio of 96.9% ) were amplified from 9 primers pairs, among which the polymorphism ratio of 4 primer combinations reached 100%. The number of alleles of different loci detected by primers ranged from 1.652 8—1.888 9, with an average of 1.710 3. The number of effective alleles from 1.182 1 to 1.306 8, with an average of 1.240 3. Gene diversity from 0.120 1 to 0.190 9, with an average of 0.152 2. Shannon index from 0.200 0 to 0.304 8, with an average of 0.245 5. The genetic similarity coefficient of the 30 samples from 0.746 4 to 0.885 3, with variation of 0.138 9, indicating that the width of the genetic background of the 30 varieties was relatively narrow. At genetic similarity coefficient of 0.796, 30 pecan varieties were divided into 3 groups. In general, the small roughy series were clustered into one as their smaller genetic similarity coefficient than that of other varieties. As good parents, they were undoubtedly worthy of further study and utilization.

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备注/Memo

备注/Memo:
收稿日期：2021-11-17修回日期：2021-12-20
基金项目：国家林业和草原局华东核桃工程技术研究中心；山东省重点研发计划项目“核桃分子辅助杂交育种及抗病优质新品种选育”（2020LZGC0901）；山东省泰山产业领军人才工程高效生态农业创新类项目“果材兼用核桃新种质创制及培育关键技术与产业化”（LJNY201805）；核桃产业国家创新联盟（NAWI）
作者简介：梁燕（1985- ），女，山东滕州人，工程师，硕士。主要研究方向：经济林育种与栽培。E-mail：272655020@qq.com
*通信作者：韩传明（1963- ），男，山东莱芜人，研究员，大学本科毕业。主要从事经济林遗传育种与栽培技术研究。E-mail：hanchuanming63@163.com
更新日期/Last Update: 2022-04-04