[1]吕运舟,窦全琴,蒋泽平.薄壳山核桃愈伤组织诱导的影响因素[J].江苏林业科技,2015,42(05):29-32.[doi:10.3969/j.issn.1001-7380.2015.05.009]
 LYU Yun-zhou,DOU Quan-qin,JIANG Ze-ping.Impact factors of the callus inducement and growth of Carya illinoensis in vitro culture[J].Journal of Jiangsu Forestry Science &Technology,2015,42(05):29-32.[doi:10.3969/j.issn.1001-7380.2015.05.009]
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薄壳山核桃愈伤组织诱导的影响因素()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第42卷
期数:
2015年05期
页码:
29-32
栏目:
试验研究
出版日期:
2015-10-30

文章信息/Info

Title:
Impact factors of the callus inducement and growth of Carya illinoensis in vitro culture
文章编号:
1001-7380(2015)05-0029-04
作者:
吕运舟窦全琴蒋泽平
江苏省林业科学研究院,江苏 南京211153
Author(s):
LYU Yun-zhou DOU Quan-qin JIANG Ze-ping
Jiangsu Academy of Forestry, Nanjing 211153,China
关键词:
薄壳山核桃组织培养愈伤组织诱导胚根胚轴
Keywords:
Carya illinoensis Tissue culture Callus Induction Radicle Hypocotyl
分类号:
S664.1;Q943.1
DOI:
10.3969/j.issn.1001-7380.2015.05.009
文献标志码:
A
摘要:
以薄壳山核桃品种马罕×波尼的杂交种子为材料,研究不同外植体来源、不同培养基成分及不同质量浓度的植物生长调节剂对愈伤组织诱导效率的影响。结果表明,MS较1/2 MS和WPM培养基更加有利于薄壳山核桃愈伤组织的诱导和继代培养;最佳植物生长调节剂组合为2.0 mg/L NAA+ 0.1 mg/L 6-BA;种子苗幼茎较胚根、胚轴、幼叶更适于作为愈伤组织诱导的起始外植体,愈伤诱导率为72.79%。
Abstract:
The hybrid seeds between ‘Mahan’ and ‘Pownee’ were used to resarch the effect of different explant, different component of media and different concentration of plant growth regulators on the efficiency of callus induction. The results showed that MS basic medium was more suitable for callus induction and subculture of Carya illinoensis,and 2.0 mg/L NAA+ 0.1 mg/L 6-BA was the optimal combination. The tender stem segments were more suitable for callus induction compared with radicle, hypocotyls and spires, with the induction rate of 72.79%.

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备注/Memo

备注/Memo:
收稿日期:2015-09-16;修回日期:2015-10-15
基金项目:江苏省林业科学研究院青年基金项目“美国薄壳山核桃组织培养研究”(JAF-2012-08);2013年江苏省林业三新工程项目“薄壳山核桃早果丰产栽培技术及模式集成示范”(lysx[2013]06)
作者简介:吕运舟(1983-),男,安徽六安人,博士,从事林木遗传育种及栽培工作。
更新日期/Last Update: 2015-10-31