[1]郑纪伟,何旭东,隋德宗,等.柳树DNA条形码的筛选与鉴定研究[J].江苏林业科技,2024,51(04):14-19.[doi:10.3969/j.issn.1001-7380.2024.04.003]
　Zheng Jiwei,He Xudong,Sui Dezong,et al.Screening and identification of DNA barcodes in willow[J].Journal of Jiangsu Forestry Science &Technology,2024,51(04):14-19.[doi:10.3969/j.issn.1001-7380.2024.04.003]
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柳树DNA条形码的筛选与鉴定研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第51卷
期数:
2024年04期
页码:
14-19
栏目:
试验研究
出版日期:
2024-08-31

文章信息/Info

Title:
Screening and identification of DNA barcodes in willow
文章编号:
1001-7380（2024）04-0014-06
作者:
郑纪伟; 何旭东; 隋德宗; 王保松
江苏省林业科学研究院，江苏 南京 211153
Author(s):
Zheng Jiwei;  He Xudong;  Sui Dezong;  Wang Baosong
Jiangsu Academy of Forestry, Nanjing 211135,China
关键词:
柳树;  DNA条形码;  筛选;  ITS;  分子鉴定
Keywords:
Salix;  DNA barcode;  Screening;  ITS; Molecular identification
分类号:
Q755；S792.12
DOI:
10.3969/j.issn.1001-7380.2024.04.003
文献标志码:
A
摘要:
柳树具观赏、生物修复、生物能源等多种功能，对其开展DNA条形码评估在种质资源保护和利用方面具有非常重要的应用价值。通过对柳树样品进行ITS、ITS2、matK、rbcL和rpoC1片段扩增和测序，结合GenBank数据，共获得柳树22个种，63份样品共计396条序列。比较了5个DNA片段的通用性、序列特征、种内和种间变异，并基于Best Match (BM)、Best Close Match (BCM)、BLAST及Neighbor Joining(NJ)4种方法评估DNA条形码在柳树中的鉴定能力。结果表明，5个DNA条形码中，ITS在柳树种扩增效率和测序成功率高，种内和种间遗传变异明显，鉴定效率最高，建议作为首选的单位点DNA条形码；3个条形码组合中，ITS+ITS2+rbcL鉴定成功率最高，为78.16%，4个DNA条形码组合中，ITS+ITS2+matK+rbcL物种鉴别成功率最高，为88.28%，建议ITS+ITS2+matK+rbcL作为柳树分子鉴定最优条形码组合。
Abstract:
DNA barcoding evaluation of willow is of great application value in germplasm resource protection and utilization, as willow showing multifunctional, integrating ornament, bioremediation and bioenergy. Through the amplification and sequencing of ITS, ITS2, matK, rbcL and rpoC1 fragments of willow samples, combined with GenBank data, we obtained 396 sequences from 22 species of willow. The comparisons of effective sequence ratios, characteristics of sequences, divergence of intra- and inter-varieties among the five DNA fragments had been performed to evaluate the recognition ability of DNA barcodes by using four methods as Best Match (BM), Best Close Match (BCM), BLAST and Neighbor Joining(NJ). The results showed that ITS, among the 5 DNA barcodes, had high amplification efficiency and sequencing success rate, obvious intraspecific and interspecific genetic variation, and the highest identification efficiency in willow species.Therefore, it was recommended to be the preferred unit point DNA barcode. Among the 3 barcode combinations, the success rate of ITS+ITS2+rbcL identification was the highest (78.16%) while among the 4 DNA barcode combinations, the success rate of ITS+ITS2+matK+rbcL identification was the highest (88.28%). Thus, it is suggested that ITS+ITS2+matK+rbcL be the best barcode combination for molecular identification of willow.

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备注/Memo

备注/Memo:
收稿日期：2024-05-19;修回日期：2024-06-27
基金项目：江苏省林业科学研究院青年基金项目“基于DNA条形码技术的柳树分子鉴定研究”（JAF-2016-01）;江苏省林业科技创新与推广项目“江苏耐盐乡土林木树种培育长期科研基地”（LYKJ[2021]08）；江苏省林业科学研究院自主科研项目“柳树专用新品种选育”（ZZKY202101）
作者简介：郑纪伟(1986- )，男，河南洛阳人，高级工程师，硕士。主要研究方向为林木遗传育种。E-mail:zjw932333@163.com
更新日期/Last Update: 2024-09-24