[1]周洁,宋雪晴,何旭东,等.柳树 SjMIPS 基因的克隆及其表达分析[J].江苏林业科技,2016,43(06):1-5.[doi:10.3969/j.issn.1001-7380.2016.06.001 ]
　ZHOU Jie,SONG Xue-qing,HE Xu-dong,et al.Molecular cloning and expression analysis of MIPS gene from Salix under salt stress[J].Journal of Jiangsu Forestry Science &Technology,2016,43(06):1-5.[doi:10.3969/j.issn.1001-7380.2016.06.001 ]
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柳树 SjMIPS 基因的克隆及其表达分析()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第43卷
期数:
2016年06期
页码:
1-5
栏目:
试验研究
出版日期:
2016-12-30

文章信息/Info

Title:
Molecular cloning and expression analysis of MIPS gene from Salix under salt stress
文章编号:
1001-7380（2016）06-0001-05
作者:
周洁1; 宋雪晴2; 何旭东1 ; 王保松1
1. 江苏省林业科学研究院， 江苏南京211153；
2. 上海市七宝德怀特高级中学，上海201101
Author(s):
ZHOU Jie1; SONG Xue-qing2;  HE Xu-dong1;  WANG Bao-song1
1.Jiangsu Academy of Forestry, Nanjing 211135，China;
2. Shanghai Qibao Dwight High School，Shanghai 201101，China
关键词:
柳树; 肌醇磷酸合成酶; 肌醇; 基因; 克隆; 盐胁迫; 表达
Keywords:
Salix sp.;  Myo-inositol phosphate synthase;  Myo-inositol;  Gene;  Cloning;  Salt Stress;  Expression
分类号:
S792.12;Q943.2
DOI:
10.3969/j.issn.1001-7380.2016.06.001
文献标志码:
A
摘要:
肌醇磷酸合成酶（MIPS）以葡萄糖-6-磷酸为合成前体，是肌醇合成过程中关键酶。MIPS生成的肌醇在耐盐植物的耐盐机制中起到渗透压调节物的作用。该研究从柳树（Salix Jiangsuensis 2345）叶片中成功克隆出MIPS基因，命名为SjMIPS。对扩增的基因进行生物信息学分析显示柳树SjMIPS基因包含1 533 bp的开放阅读框，编码1个511个氨基酸的蛋白质。蛋白质结构域分析显示SjMIPS基因含有4个保守的结构域，结构域1（GWGGNNG），结构域2(LWTANTERY)，结构域3(NGSPQNTFVPG)和结构域4（SYNHLGNNDG），属于典型的MIPS基因。系统进化树分析表明柳树SjMIPS基因与杨树PtMIPS基因亲缘关系较近。实时荧光定量PCR结果显示柳树SjMIPS基因在171 mM NaCl盐胁迫处理24—48 h后上调，表明柳树MIPS基因是盐胁迫诱导型基因。该研究表明柳树SjMIPS基因为盐胁迫应答基因，在参与肌醇的生物合成、调节渗透压方面起着重要作用。
Abstract:
Myo-inositol phosphate synthase (MIPS) is the key enzyme of myo-inositol synthesis, using glucose-6-phosphate as the initial precursor. MIPS producing myo-inositol plays a pivotal role in protection mechanisms in salt-tolerant plant species. In this research, a MIPS gene was cloned from salt-treated leaves of Salix Jiangsuensis 2345 and named SjMIPS. SjMIPS contained a 1 533 bp of the largest open reading frame，encoding 511 amino acids. Protein alignment showed SjMIPS contained four complete conserved domains, Domain 1(GWGGNNG)，Domain 2(LWTANTERY)，Domain 3 （NGSPQNTFVPG） and Domain 4(SYNHLGNNDG). Phylogenetic analysis showed that SjMIPS was closer to MYBs from Populus trichocarpa. Real-time PCR analysis showed SjMIPS was in response to salt stress after treated with 171mM NaCl for 24, 48 h, indicating that SjMIPS was responsive to salt. It was concluded that SjMIPS was a salt responsive gene and its expression product might function as compatible solute for protection against salt stress in Salix.

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备注/Memo

备注/Memo:
收稿日期：2016-10-11;修回日期：2016-10-27
江苏省自然科学基金项目“柳树盐胁迫响应基因表达谱分析和重要耐盐基因鉴定”（BK20141039）；国家自然科学基金项目“灌木柳盐胁迫基因表达谱的解析和重要耐盐基因的鉴定”（31400572）；江苏省科技支撑（农业）项目“耐盐高生物量灌木柳新品种选育及高效栽培技术研究”（BE2013449）
作者简介：周洁（1986- ），女，江苏宜兴人，助理研究员，博士。主要从事林木分子育种研究。
更新日期/Last Update: 2016-12-30