[1]陈凌艳,陈拓,荣俊冬,等.雷公藤组织培养快速繁殖技术研究[J].江苏林业科技,2016,43(02):8-12.[doi:10.3969/j.issn.1001-7380.2016.02.003]
　CHEN Ling-yan,CHEN Tuo,RONG Jun-dong,et al.Tissue culture and rapid micropropagation of tripterygium wilfordii[J].Journal of Jiangsu Forestry Science &Technology,2016,43(02):8-12.[doi:10.3969/j.issn.1001-7380.2016.02.003]
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雷公藤组织培养快速繁殖技术研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第43卷
期数:
2016年02期
页码:
8-12
栏目:
试验研究
出版日期:
2016-04-30

文章信息/Info

Title:
Tissue culture and rapid micropropagation of tripterygium wilfordii
文章编号:
1001-7380（2016）02-0008-05
作者:
陈凌艳1; 陈拓1; 荣俊冬2; 何天友1; 郑郁善2*
1. 福建农林大学园林学院，福建 福州350002；
2. 福建农林大学工业原料林研究所，福建 福州 350002
Author(s):
CHEN Ling-yan1;  CHEN Tuo1; RONG Jun-dong2; HE Tian-you1; ZHENG Yu-shan2*
1. College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Institute of Industrial Forest, Agriculture and Forestry University, Fuzhou 350002, China
关键词:
雷公藤; 组织培养; 快速繁殖; 增殖
Keywords:
Tripterygium wilfordii;  Tissue culture;  Rapid micropropagation;  Proliferation
分类号:
Q949.754.7;R282.2
DOI:
10.3969/j.issn.1001-7380.2016.02.003
文献标志码:
A
摘要:
以雷公藤带芽幼嫩茎段为外植体，通过不同的培养基配方，对雷公藤组织培养快速繁殖体系建立进行了研究。试验结果表明：外植体经75%酒精浸泡30 s后，用无菌水冲洗3遍，0.1%升汞溶液浸泡5 min，无菌水冲洗5遍的消毒效果最好，雷公藤组织培养苗的污染率为7.8%；最佳诱导培养基为MS+0.05 mg/L NAA +0.5 mg/L 6-BA，平均速度为3.2 d；最佳继代增殖培养基为1/2 MS+2.0 mg/L 6-BA +1.0 mg/L NAA +25 g/L蔗糖，月增殖系数达5.83；最佳生根培养基为 1/2 MS+2.0 mg/L IBA +0.5 g/L AC培养基，生根率达78.3％；最有利于移栽的基质为等容积的沙子+红壤土+泥炭土混合基质，成活率高达87.31%。
Abstract:
By means of different culture media, a tissue culture system of Tripterygium wilfordii was established with its new shoot with buds as explant. Experimental results showed that the most suitable procedure for sterilization was to soak the explants into 0.1% HgCl2 solution for 5 min and then wash with sterile water for 5 times after soaking them into 75% alcohol for 30 s and washing with sterile water for 3 times. The optimum initial medium was MS supplemented with 0.05 mg/L NAA +0.5 mg/L 6-BA , the optimum subculture medium was 1/2 MS with 2.0 mg/L 6-BA +1.0 mg/L NAA + 25 g/L sucrose, and the optimum rooting medium was 1/2 MS with 2.0 mg/L IBA +0.5 g/L AC. The plantlets reached 87.31% survival rate after transplanted in the substrate containing equal volume of sand, red loam and peat soil.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2016-03-29;修回日期：2016-04-02
基金项目：国家科技支撑计划项目“雷公藤短葶山麦冬GAP关键技术研究”(2009BAI73B00); 福建省中药材GAP工程技术研究中心资助项目“福建省中药材GAP示范基地的建设”(2008Y2001);福建省科技重大专项基金资助项目“福建省中药材GAP工程技术研究中心”(2004YZ02-05)
作者简介：陈凌艳（1986- ），女，福建福州人,讲师。从事园林植物与观赏园艺研究。
*通信作者：郑郁善（1960- ），男，福建永泰人，教授。博士生导师，从事森林培育学研究。
更新日期/Last Update: 2016-05-16