[1]曾丽琼,何学友,蔡守平,等.具杀松材线虫活性细菌的筛选和鉴定[J].江苏林业科技,2018,45(02):6-9.[doi:10.3969/j.issn.1001-7380.2018.02.002]
　ZENG Li-qiong,HE Xue-you,CAI Shou-ping,et al.Selection and identification of the bacterium with nematicidal activity to Bursaphelenchus xylophilus[J].Journal of Jiangsu Forestry Science &Technology,2018,45(02):6-9.[doi:10.3969/j.issn.1001-7380.2018.02.002]
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具杀松材线虫活性细菌的筛选和鉴定()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第45卷
期数:
2018年02期
页码:
6-9
栏目:
试验研究
出版日期:
2018-04-30

文章信息/Info

Title:
Selection and identification of the bacterium with nematicidal activity to Bursaphelenchus xylophilus
文章编号:
1001-7380（2018）01-0006-04
作者:
曾丽琼; 何学友; 蔡守平; 黄金水
福建省林业科学研究院/国家林业局南方山地用材林培育重点实验室，福建福州350012
Author(s):
ZENG Li-qiong; HE Xue-you; CAI Shou-ping; HUANG Jin-shui
Fujian Academy of Forestry Sciences, The Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fuzhou 350012, China
关键词:
松材线虫; 细菌; 杀线虫活性; 16S rDNA
Keywords:
Bursaphelenchus xylophilus; Bacterium;  Nematicidal activity; 16S rDNA
分类号:
S763.306.4；S767.3+2
DOI:
10.3969/j.issn.1001-7380.2018.02.002
文献标志码:
A
摘要:
为筛选出对松材线虫具有抑制作用的生物防治细菌，对马尾松内生细菌进行分离纯化，从中筛选出6株对松材线虫具有杀线虫活性的细菌，其中细菌Z11-2和细菌G36具有较强的杀线活性，用它们的培养滤液分别处理松材线虫24 h，松材线虫的死亡率分别达92%和89%，48 h后线虫消解率分别是87.4%和77.5%。通过细菌形态特征观察和16S rDNA测序及其系统发育分析，菌株Z11-2和G36与短小芽孢杆菌Bacillus pumilus处于同一分支，同源性分别为100%和99%，初步鉴定细菌Z11-2和G36为短小芽孢杆菌。
Abstract:
Six endophytic bacterial strains were separated from the Pinus massoniana twigs. The bioassay result showed that the mortality of Bursaphelenchus xylophilus treated for 24 h with Z11-2 strain and G36 strain were 92% and 89% respectively, with the decomposition rate 87.4% and 77.5% respectively for 48 h's treatment. By combination of the morphologic observation with 16S rDNA sequence analysis, according to the position in phylogenetic tree, we found that Z11-2 strain and G36 strain were identified as Bacillus pumilus, as showing 100% and 99% homology respectively.

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备注/Memo

备注/Memo:
收稿日期：2018-01-23;修回日期：2018-03-29
基金项目：福建省自然科学基金计划项目“松材线虫病生防细菌的研究”（2015J01096）；福建省林业科学研究项目“松材线虫生防细菌的筛选研究”（闽林科[2014]2号）
作者简介：曾丽琼(1984- )，女，福建泉州人，工程师，硕士。从事森林病虫害防治与研究。
更新日期/Last Update: 2018-05-23