[1]康德星,王晓禹,梅 梅,等.3种槭属植物花粉离体萌发适宜条件及活力检测方法[J].江苏林业科技,2018,45(01):5-9.[doi:10.3969/j.issn.1001-7380.2018.01.002]
　KANG De-xing,WANG Xiao-yu,MEI Mei,et al.Conditions for in vitro germination and testing method for pollen viability of 3 species maple[J].Journal of Jiangsu Forestry Science &Technology,2018,45(01):5-9.[doi:10.3969/j.issn.1001-7380.2018.01.002]
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3种槭属植物花粉离体萌发适宜条件及活力检测方法()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第45卷
期数:
2018年01期
页码:
5-9
栏目:
试验研究
出版日期:
2018-02-28

文章信息/Info

Title:
Conditions for in vitro germination and testing method for pollen viability of 3 species maple
文章编号:
1001-7380（2018）01-0005-05
作者:
康德星; 王晓禹; 梅 梅 ; 张晓林; 陆秀君*
沈阳农业大学，辽宁 沈阳 110866
Author(s):
KANG De-xing;  WANG Xiao-yu;  MEI Mei;  ZHANG Xiao-lin;  LU Xiu-jun*
Shenyang Agriculture University, Shenyang 110866 ,China
关键词:
槭属植物; 花粉离体萌发; 过氧化物酶法; 亚甲基蓝法; 花粉活力测定
Keywords:
Acer sp.;  In vitro pollen germination;  Peroxidase solution;  Methylene blue staining;  Pollen viability test
分类号:
Q944.42；S792.35
DOI:
10.3969/j.issn.1001-7380.2018.01.002
文献标志码:
A
摘要:
该文以红花槭、元宝枫、糖槭为试验材料，蕾期采集花粉，通过不同培养温度、不同蔗糖和硼酸质量浓度处理后进行花粉离体萌发，筛选其最佳萌发条件。采用TTC法、I2-IK法、过氧化物酶法、亚甲基蓝法以及离体萌发法对3种槭属植物进行活力测定，探讨其有效的活力测定方法。结果表明：红花槭花粉在培养温度28 ℃，蔗糖75 g/L+硼酸150 mg/L的培养基中萌发率最高为81.95%；元宝枫花粉在培养温度25 ℃，蔗糖100 g/L+硼酸250 mg/L的培养基中萌发率最高为66.7%；糖槭花粉在培养温度28 ℃，蔗糖25 g/L+硼酸350 mg/L的培养基中萌发率最高为88.37%。过氧化物酶法与亚甲基蓝法活力测定效果较好，与离体萌发相比差异显著，其余2种染色剂不适合3种槭属植物花粉染色。
Abstract:
With Acer rubrum L., A. truncatum Bunge and A. saccharumMarsh. taken as test materials, their pollen was germinated in vitro under the conditions with different temperatures, different concentrations of sucrose and boric acid, and finally, the suitable pollen germination condition for each maple species was conformed. TTC method, I2-IK method, peroxidase solution, methylene blue staining and in vitro pollen germination method were used to detect the pollen viability of these maple species, and the effectiveness of these methods was assessed. The result showed that A. rubrum pollen at 28 ℃ in the culture medium containing 75 g/L sucrose and 150 mg/L boric acid had a germination rate of 81.95%; A. truncatum Bunge pollen at 25 ℃ in the culture medium supplemented with 100 g/L sucrose and 250 mg/L boric acid had a germination rate of 66.7%; A. saccharumMarsh. pollen at 28 ℃ in the culture medium with 25 g/L sucrose and 350 mg/L boric acid had a germination rate of 88.37%. There were significant differences among the pollen viabilities detected by peroxidase solution, methylene blue staining and in vitro pollen germination, but neither TTC method nor I2-IK staining method was suitable for pollen viability test of these maple species.

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备注/Memo

备注/Memo:
收稿日期：2017-10-29;修回日期：2017-11-17
基金项目：辽宁省农业科技攻关项目“经济林、景观林种质创新及新品种引进筛选”（No.2015207005）
作者简介：康德星（1992- ），男，山西朔州人，硕士研究生。从事森林培育研究。E-mail:1160433940@qq.com。
*通信作者：陆秀君（1966- ），女，辽宁铁岭人，教授，博士。从事森林培育研究。E-mail：Lxjsyau@126.com。
更新日期/Last Update: 2018-04-02