[1]黄婧,周鹏,李 飞,等.小果冬青组织培养和瓶外生根关键因子研究[J].江苏林业科技,2024,51(04):8-13.[doi:10.3969/j.issn.1001-7380.2024.04.002]
　Huang Jing,Zhou Peng,Li Fei,et al.Key factors for tissue culture and ex vitro rooting of Ilex micrococca[J].Journal of Jiangsu Forestry Science &Technology,2024,51(04):8-13.[doi:10.3969/j.issn.1001-7380.2024.04.002]
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小果冬青组织培养和瓶外生根关键因子研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第51卷
期数:
2024年04期
页码:
8-13
栏目:
试验研究
出版日期:
2024-08-31

文章信息/Info

Title:
Key factors for tissue culture and ex vitro rooting of Ilex micrococca
文章编号:
1001-7380（2024）04-0008-06
作者:
黄婧; 周鹏; 李 飞; 陈亚辉; 张敏*
江苏省林业科学研究院， 江苏 南京 211153
Author(s):
Huang Jing;  Zhou Peng;  Li Fei;  Chen Yahui; Zhang Min*
Jiangsu Academy of Forestry, Nanjing 211153,China
关键词:
小果冬青; 组织培养; 增殖系数; 瓶外生根; 关键因子
Keywords:
Ilex micrococca; Tissue culture; Proliferation coefficient; Ex vitro rooting; Key factor
分类号:
Q943.1；S792.99
DOI:
10.3969/j.issn.1001-7380.2024.04.002
文献标志码:
A
摘要:
为建立小果冬青组织培养高效繁育技术体系，该研究分析了诸多因子对组织培养苗增殖及其瓶外生根情况的影响。通过正交试验，研究生长调节剂种类、质量浓度和蔗糖含量对小果冬青苗增殖系数的影响以及生根剂种类、质量浓度、处理时间和基质类型对小果冬青组织培养苗瓶外生根的影响。结果表明：影响小果冬青茎段芽增殖的主要因素为6-BA和蔗糖含量，较佳配比方案：6-BA质量浓度为0.5 mg/L，NAA质量浓度为0.1 mg/L，蔗糖含量为40 g/L，此时小果冬青组织培养苗增殖系数为4.52。影响小果冬青瓶外生根效果的主次顺序为基质类型、生根剂处理时间、生根剂质量浓度、生根剂种类。选用生根剂种类为IBA，质量浓度为200 mg/L，处理时间为1 min，基质为泥炭，此时小果冬青组织培养苗瓶外生根率为84.44%，生根数5.36，平均根长3.78 cm。
Abstract:
To establish an efficient propagation system for Ilex micrococca tissue culture, the effects of key factors on the proliferation of tissue culture seedlings and the ex vitro rooting of micro-shoots were analyzed. We studied the effects of exogenous hormone type, concentration, and sucrose content on the proliferation coefficient, and the effects of rooting agent type, concentration, processing time, and substrate type on the ex vitro rooting. The results showed that the dominant factors affecting the proliferation were 6-BA concentration and sucrose concentration. The optimal solution was 6-BA concentration of 0.5 mg/L, NAA concentration of 0.1 mg/L, and sucrose concentration of 40 g/L. Under this condition, the proliferation coefficient was 4.52. The factors that affected the ex vitro rooting effect were, inoder, substrate type, agent processing time, concentration and agent type. The preferable conditions of ex vitro rooting of the micro-shoots were that after pretreated with IBA concentration 200 mg/L, processing time 1 minute in the peat substrate, 84.44% of rooting rate, 5.36 of average root number and 3.78 cm of average root length could be gotten.

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备注/Memo

备注/Memo:
收稿日期：2024-05-09;修回日期：2024-06-07
基金项目：江苏省林业科技创新与推广项目“小果冬青工厂化繁育技术研究示范”（LYKJ[2022]15）
作者简介：黄婧（1987- ），女，江苏镇江人，高级工程师，博士。主要从事林木花卉繁育技术研究。
*通信作者：张敏（1980- ），女，内蒙古乌海人，研究员，博士。主要从事生物技术与林木花卉良种繁育工作。
更新日期/Last Update: 2024-09-24