[1]杨甜,何炎红*,贾文宝,等.蕤核种子诱导芽及快速繁殖体系建立[J].江苏林业科技,2023,50(06):16-20.[doi:10.3969/j.issn.1001-7380.2023.06.003]
　Yang Tian,He Yanhong*,Jia Wenbao,et al.Establishment of seed-induced bud and rapid micro-propagation system of Prinsepia uniflora Batal[J].Journal of Jiangsu Forestry Science &Technology,2023,50(06):16-20.[doi:10.3969/j.issn.1001-7380.2023.06.003]
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蕤核种子诱导芽及快速繁殖体系建立()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第50卷
期数:
2023年06期
页码:
16-20
栏目:
试验研究
出版日期:
2023-12-31

文章信息/Info

Title:
Establishment of seed-induced bud and rapid micro-propagation system of Prinsepia uniflora Batal
文章编号:
1001-7380（2023）06-0016-05
作者:
杨甜1; 何炎红1*; 贾文宝1; 田春2; 林涛1
1.内蒙古农业大学林学院，内蒙古 呼和浩特 010019；
2.内蒙古自治区中医医院，内蒙古 呼和浩特 010010
Author(s):
Yang Tian1; He Yanhong1*;  Jia Wenbao1; Tian Chun2; Lin Tao1
1.Forestry College of Inner Mongolia Agricultural University, Hohhot 010019,China;
2. Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine, Hohhot 010010, China
关键词:
蕤核; 成熟种子; 组织培养; 快速繁殖
Keywords:
Prinsepia uniflora Batal.;  Mature seed;  Tissue culture;  Rapid propagation
分类号:
Q943.1；S793.9
DOI:
10.3969/j.issn.1001-7380.2023.06.003
文献标志码:
A
摘要:
以蕤核成熟种子为外植体，对外植体的消毒方法、芽的诱导、增殖培养以及生根培养进行研究，以建立蕤核组织培养快速繁殖体系，使其在短期内大量萌发和快速扩大繁殖。结果表明：蕤核种子在诱导芽时，种皮会阻碍种子萌发，降低其萌发速率并提高感染率，故需要在接种前剥离种皮；75%酒精消毒30 s后搭配0.5%的NaClO消毒15 min为最佳消毒方案，感染率为2.6%，萌发率为97.07%；最适诱导培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA，诱导率为39.5%；最适继代培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA+0.1 mg/L IBA，增殖系数达到2.83；最适生根培养基为1/2 MS+1.0 mg/L IBA+0.3 g/L活性炭，生根率为95%；通过种子诱导得到再生植株，成功建立蕤核组织培养快速繁殖体系，为其遗传改良及科学规模化生产提供一定的理论基础和技术支撑。
Abstract:
In this experiment, the mature seeds of Prinsepia uniflora were used as explants to study the disinfection method, bud induction, proliferation and rooting culture, so as to establish a rapid propagation system. The results showed that the seed coat would hinder the seed germination, reduce the germination rate and increase the infection rate, so it was necessary to peel the seed coat before inoculation. The best disinfection scheme was 75 % alcohol disinfection for 30 s followed by 0.5 % NaClO disinfection for 15 min. The infection rate was 2.6 % and the germination rate was 97.07 %. The optimum induction medium was MS+ 1.0 mg/L 6-BA+0.3 mg/L NAA, and the induction rate was 39.5 %. The optimal subculture medium was MS+1.0 mg/L 6-BA+0.3 mg/L NAA+0.1 mg/L IBA, and the proliferation coefficient reached 2.83. The optimum rooting medium was 1/2 MS+1.0 mg/L IBA+0.3 g/L activated carbon, and the rooting rate could get 95 %. The regeneration plant was obtained by seed induction, and rapid propagation system of P. uniflora was successfully established for its genetic improvement and scientific large-scale production.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2023-09-10;修回日期：2023-10-23
基金项目：内蒙古自治区项目“特色林果‘蒙杏1 号’西伯利亚杏品种示范与推广”（2022YFXZ0024）；“寒旱区主栽果树种质创新及新品种选育”（2021GG0034）
作者简介：杨甜(1998- )，女，内蒙古巴彦淖尔人，硕士研究生。主要研究方向：森林培育。E-mail：1594273606@qq.com
*通信作者：何炎红(1979- )，女，内蒙古呼和浩特人，副教授，博士，硕士生导师。主要研究方向：森林培育理论与技术。E-mail：hyh20012008@imau.edu.cn
更新日期/Last Update: 2024-03-14