[1]吕 铭,王肖雄,何汝峰,等.红叶紫薇“一步法”快速繁殖试验[J].江苏林业科技,2017,44(03):15-18.[doi:10.3969/j.issn.1001-7380.2017.03.004]
 LYU Ming,WANG Xiao-xiong,HE Ru-feng,et al.Micro-propagation of Red Crape Myrtle[J].Journal of Jiangsu Forestry Science &Technology,2017,44(03):15-18.[doi:10.3969/j.issn.1001-7380.2017.03.004]
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红叶紫薇“一步法”快速繁殖试验()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第44卷
期数:
2017年03期
页码:
15-18
栏目:
试验研究
出版日期:
2017-06-30

文章信息/Info

Title:
Micro-propagation of Red Crape Myrtle
文章编号:
1001-7380(2017)03-0015-04
作者:
吕 铭1王肖雄2何汝峰1胡恒康1张启香1*
1. 浙江农林大学 浙江 临安 31100;
2. 宁波林丰种业科技有限公司 浙江 宁波 315601
Author(s):
LYU Ming1WANG Xiao-xiong2HE Ru-feng1HU Heng-kang1ZHANG Qi-xiang1*
1. Zhejiang A & F University, Lin’an 311300, China;
2. Ningbo Linfeng Seed Industry Co. Ltd., Ningbo 315601, China
关键词:
红叶紫薇快速繁殖植物生长调节剂
Keywords:
Lagerstroemia indica Pink VelourRapid micro-propagationPlant growth regulator
分类号:
S723.1+32;S685.99
DOI:
10.3969/j.issn.1001-7380.2017.03.004
文献标志码:
A
摘要:
以红叶紫薇当年生新枝具腋芽茎段为外植体材料,研究其灭菌时间长短对外植体污染率及腋芽萌发率的影响,根据基本培养基上其离体腋芽萌发诱导情况,对培养基(MS,1/2 MS,WPM,1/2 WPM)进行筛选,并根据植物生长调节剂(BA,NAA,KT)及其质量浓度的不同配合,筛选出无菌苗“一步法”增殖、生根培养基。试验结果显示:使用0.1%HgCl2对外植体灭菌12 min,能在保证低污染率(13.33%)的同时,获得较高的腋芽萌发率(80.00%);1/2 MS培养基上的腋芽萌发率(96.67%)和腋芽长度(1.60 cm)均显著高于其他3种培养基;在1/2 MS+1.0 mg/L BA+0.1mg/L KT培养基中,无菌芽可同时增殖及生根,芽增殖系数(4.67)、苗高(2.53 cm)、生根率(100%)及根数(4.33条)均显著高于其他处理。
Abstract:
The annual shoots of Red Crape Myrtle were used as explant for in vitro micro-propagation. Different sterilization manners, basal media and plant growth regulators were selected for one-step multiplication. The results showed that HgCl2 sterilization for 12 min could keep explant in low contamination percentage but high germination rate. The germination rate (up to 96.67%) of axillary bud and bud length (1.6 cm) of explant cultured on 1/2MS basal medium were significantly higher than those on MS, WPM and 1/2MS. The in vitro shoots could grow and multiply in one-step. Shoot length (up to 2.53 cm), root percentage (100%) and root number (4.33) of in vitro shoots cultured on 1/2MS supplemented with 1.0 mg/L BA, 0.1 mg/L KT were significantly higher than those on other treatments. And the multiplication coefficient could also reach 4.67.

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备注/Memo

备注/Memo:
收稿日期:2017-04-29;修回日期:2017-05-17
基金项目:浙江省大学生新苗人才计划项目“红叶紫薇快繁关键技术的研究”(2016R412024)
作者简介:吕 铭(1995- ),男,浙江桐乡人,大学本科。主要从事观赏植物组织培养研究工作。
*通信作者:张启香(1975- ),女,江苏南京人,博士,教授。主要从事植物组织培养和发育生物学研究工作。
更新日期/Last Update: 2017-07-19