[1]刘学松,邵慰忠,邓创,等.丛枝菌根真菌增强柏木幼苗抗干旱性研究[J].江苏林业科技,2024,51(06):1-6.[doi:10.3969/j.issn.1001-7380.2024.06.001]
　Liu Xuesong,Shao Weizhong,Deng Chuang,et al.Arbuscular mycorrhizal fungi can enhance the drought resistance of the seedlings of Cupressus funebris[J].Journal of Jiangsu Forestry Science &Technology,2024,51(06):1-6.[doi:10.3969/j.issn.1001-7380.2024.06.001]
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丛枝菌根真菌增强柏木幼苗抗干旱性研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第51卷
期数:
2024年06期
页码:
1-6
栏目:
试验研究
出版日期:
2024-12-31

文章信息/Info

Title:
Arbuscular mycorrhizal fungi can enhance the drought resistance of the seedlings of Cupressus funebris
文章编号:
1001-7380（2024）06-0001-06
作者:
刘学松1; 邵慰忠1; 邓创2; 3 ; 江波3; 王志高3*
1.建德市林业局，浙江 杭州 311600；
2.浙江农林大学林业与生物技术学院，浙江 杭州 311300；
3.浙江省林业科学研究院，浙江 杭州 310013
Author(s):
Liu Xuesong1; Shao Weizhong1; Deng Chuang2; 3;  Jiang Bo3; Wang Zhigao3*
1.Jiande Forestry Bureau,Hangzhou 311600,China;
2.College of Forestry and Biotechnology,Zhejiang A & F University,Hangzhou 311300,China;
3.Zhejiang Academy of Forestry, Hangzhou 310013,China
关键词:
丛枝菌根真菌; 干旱胁迫; 柏木; 生长; 生理
Keywords:
Arbuscular mycorrhizal fungi; Drought stress; Cupressus funebris; Growth; Physiology
分类号:
Q948.12+2.2；Q949.32；S723.9
DOI:
10.3969/j.issn.1001-7380.2024.06.001
文献标志码:
A
摘要:
丛枝菌根真菌与植物形成共生体，有助于提高植物对环境的适应能力。通过对柏木进行摩西斗管囊霉（Fm）、幼套近明囊霉（Ce）和根内根孢囊霉（Ri）3种菌剂单独接种及其等量混合接种处理（MIX），测定和分析了连续干旱胁迫过程中柏木生长和生理指标变化。结果表明，混合接种组根系浸染率显著高于单菌种接种，分别是接种Fm、Ce和Ri的1.63，1.91，1.23倍；连续干旱胁迫下丛枝菌根真菌接种显著提高了柏木的株高和根系生长，混合接种处理组的株高增长量，根尖数和根表面积分别是CK的4.36，2.36，2.47倍；接种Fm，Ri，Ce的根表面积分别是CK的109.91%，131.90%，132.99%。丛枝菌根真菌处理提高了柏木的总叶绿素含量4.17%—9.13%、叶绿素a/b值平均提高6.74%；降低了干旱胁迫过程中细胞的伤害，MDA分别为CK的82.06%—94.06%，细胞保护性酶和渗透调节物质含量显著提升，POD含量分别为CK的116.58%—138.23%，SP含量分别为CK的104.56%—147.50%。结果显示，丛枝菌根真菌处理，尤其是混合接种处理，可以显著提高柏木对干旱胁迫的适应性，促进苗木生长，为干旱条件下的柏木造林提供了新的方法。
Abstract:
Arbuscular mycorrhizal fungi (AMF) can establish symbiotic relationships with plants, significantly enhancing their capacity of adaptation to environmental conditions.Three AMFs, including Funneliformis mosseae(Fm),Claroideoglomus etunicatum（Ce）,Rhizophagus intraradices（Ri）were separately inoculated on the root of Cupressus funebris and a mixed inoculation treatment (MIX) was also set up by mixing the three AMFs in equal quantities. The growth and physiological indicators of C. funebris under continuous drought stress were measured and compared. The results showed that the root colonization rate of the mixed inoculation group was significantly higher than that of the single inoculation groups, being 1.63,1.91 and 1.23 times that of the Fm, Ce, and Ri groups, respectively. Under the continuous drought stress, AMF inoculation could significantly improved the growth of C. funebris, with the growth by the mixed inoculation group being 4.36 times of CK in plant height, 2.36 times in root tip number and 2.47 times in root surface area. The root surface area caused by Fm, Ri, and Ce was 9.91%, 31.10%， and 32.99% larger than CK. The total chlorophyll content of C. funebris was increased by 4.17% to 9.13% by AMF treatment, and the average value of chlorophyll a/b was increased by 6.74%. The AMF treatment reduced cell damage during drought stress, with the MDA being 82.06% to 94.06% of that of CK. The content of protective enzymes and osmotic adjustment substances was significantly increased, with POD being 116.58% to 138.23% of that of and SP (soluble proteins) being 104.56% to 147.50% of that of CK. This study showed that that treatment with AMF, especially mixed inoculation, could significantly enhance the adaptability of C. funebris to drought stress and promote seedling growth.

参考文献/References:

[1]季孔庶,孙志勇,方彦.林木抗旱性研究进展[J].南京林业大学学报(自然科学版),2006,30 (6):123-128.

[2]谭梦迪,冯天骄,王 平,等.近30年微生物菌剂改良土壤的研究进展[J].土壤学报, 2024, 11(7): 1-20.
[3]SMITH S E,SMITH F A.Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystem scales[J].Annual Review of Plant Biology,2011,62(1) : 227-250.
[4]刘婷.菌根真菌提高林木抗旱性研究进展[J].内蒙古农业大学学报（自然科学版）,2018,29(31):93-98.
[5]刘娜,赵泽宇,姜喜铃,等.菌根真菌提高植物抗旱性机制的研究回顾与展望[J].菌物学报,2021,40(4):851-872.
[6]贺琴,李钢铁,麻云霞.干旱胁迫下接种 AM 真菌对文冠果幼苗根系的影响[J].内蒙古农业大学学报(自然科学版),2024,11(7): 1-13.
[7]张中峰,张金池,徐广平,等.接种菌根真菌对青冈栎水分吸收的贡献[J].生态学杂志,2018,37(8):2285-2292.
[8]张月欣,麻云霞,马秀枝,等.接种 4 种丛枝菌根真菌对榆树幼苗抗旱性的影响[J].中国水土保持科学(中英文),2024,22(3):146-153.
[9]杜宣瑾,朱源,张露,等.接种丛枝菌根真菌对毛红椿幼苗生长及抗旱性的影响[J].森林与环境学报, 2024, 44(1): 71-78.
[10]栗晗,江尚焘,彭海英,等.接种土著和外源 AM 真菌对杜梨抗旱性的影响及其适应机制[J].中国农业科学,2024, 57(1): 159-172.
[11]周琳.柏木优树子代遗传分析及优良家系评选[D].雅安：四川农业大学,2017.
[12]郑 一,张振,金国庆,等.低肥力土壤施用氮磷钾肥影响柏木家系根系发育和养分吸收对钙肥的响应[J].植物营养与肥料学报,2020,26(8): 1501-1512.
[13]AALIPOUR H,NIKBAKHT A, ETEMADI N, et al. Biochemical response and interactions between arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria during establishment and stimulating growth of Arizona cypress (Cupressus arizonica G.) under drought stress[J]. Scientia Horticulturae, 2020, 261: 108923.
[14]OUAHMANE L, HAFIDI M,THIOULOUSE J,et al. Improvement of Cupressus atlantica Gaussen growth by inoculation with native arbuscular mycorrhizal fungi[J]. Journal of Applied Microbiology, 2007,103(3): 683-690.
[15]ZARIK L,MEDDICH A,HIJRI M,et al.Use of arbuscular mycorrhizal fungi to improve the drought tolerance of Cupressus atlantica G[J]. Comptes Rendus Biologies,2016,339(6):185-196.
[16]刘润进,陈应龙.菌根学[M].北京:科学出版社, 2007.
[17]YAN Q X , LI X Y, XIAO X F,et al. Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physiobiochemical responses[J].Ecology and Evolution, 2022,12(7):e9091.
[18]唐超,李敏,刘永举,等.单一及复合 AM 真菌初侵染对番茄苗的生理影响[J].中国农学通报,2013, 29(13): 114-119.
[19]张可可,蒋德明,余海滨,等.接种菌根菌剂对科尔沁沙地4种造林幼苗生长特性的影响[J].生态学杂志,2017,36(7): 1791-1800.
[20]赵祥,曾广萍,杨盼.等. AM 真菌对红花生长及其有效成分的影响[J].干旱区研究,2019,36(4): 935-942.
[21]方静,武帅,孟昭军,等.两种丛枝菌根真菌复合接种对青山杨生长生理指标的影响[J].东北林业大学学报,2024,52(4): 17-22.
[22]张华,孙纪全,包玉英.丛枝菌根真菌影响植物次生代谢产物的研究进展[J].农业生物技术学报,2015,23(8):1093-1103.
[23]唐承财,钟全林,王健.林木抗旱生理研究进展[J].世界林业研究,2008,21 (1):20-26.
[24]王福祥,肖开转,姜身飞,等.干旱胁迫下植物体内活性氧的作用机制[J].科学通报,2019, 64(17): 1765-1779.
[25]黎燕琼,郑绍伟,陈泓,等.林木抗旱性研究及其进展[J].世界林业研究,2007,20 (1):10-15.
[26]王紫瑄.丛枝菌根真菌对蒙古沙冬青根系及幼苗生长的影响: [D].晋中:山西农业大学,2023.
[27]邓创,王志高,朱锦茹,等.丛枝菌根接种对黄檀的抗旱性影响[J].分子植物育种,2024,22(15): 5107-5114.
[28]刘锦春,钟章成.水分胁迫和复水对石灰岩地区柏木幼苗根系生长的影响[J].生态学报,2009,29(12):6439-6445.
[29]付佳琳,蔡江平,刘贺永,等.内蒙古草甸草原植物叶片光合色素对极端干旱的响应[J].中国草地学报,2022,44(4):40-47.

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备注/Memo

备注/Memo:
收稿日期：2024-08-01;修回日期：2024-09-04
基金项目：浙江省与中国林业科学研究院省院合作林业科技项目“浙江省困难立地造林关键技术研究与示范” (2021SY08);浙江省“尖兵”“领雁”计划项目“受损森林生态系统快速修复和功能提升技术”(2022C02053)
作者简介：刘学松（1969- ），男，浙江建德人，高级工程师。主要从事森林培育研究。
*通信作者：王志高（1978- ），男，安徽长丰人，副研究员，博士。主要从事森林生态和森林培育研究。
更新日期/Last Update: 2025-01-23