[1]于菲,周鹏,江灿,等.响应面法优化乌饭树原花青素提取[J].江苏林业科技,2026,53(02):19-25.[doi:10.3969/j.issn.1001-7380.2026.02.003]
　Yu Fei,Zhou Peng,Jiang Can,et al.Response surface methodology optimization of proanthocyanidin extraction from Vaccinium bracteatum[J].Journal of Jiangsu Forestry Science &Technology,2026,53(02):19-25.[doi:10.3969/j.issn.1001-7380.2026.02.003]
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响应面法优化乌饭树原花青素提取

《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第53卷
期数:
2026年02期
页码:
19-25
栏目:
试验研究
出版日期:
2026-04-30

文章信息/Info

Title:
Response surface methodology optimization of proanthocyanidin extraction from Vaccinium bracteatum
文章编号:
1001-7380（2026）02-0019-07
作者:
于菲1; 2; 周鹏2; 江灿1; 2; 张强1; 张敏2*
1. 南京林业大学,江苏 南京 210037；
2.江苏省林业科学研究院，江苏 南京 211153
Author(s):
Yu Fei1; 2; Zhou Peng2;  Jiang Can1; 2;  Zhang Qiang1;  Zhang Min2*
1. Nanjing Forestry University, Nanjing 210037,China;
2.Jiangsu Academy of Forestry, Nanjing 211153,China
关键词:
乌饭树; 原花青素; 超声辅助法; 提取; 响应面法
Keywords:
Vaccinium bracteatum;  Proanthocyanidin;  Ultrasonic-assisted extraction;  Extraction;  Response surface methodology
分类号:
Q946.83+6；R284.2；S793.9
DOI:
10.3969/j.issn.1001-7380.2026.02.003
文献标志码:
A
摘要:
以乌饭树叶片为材料，采用超声辅助法提取总原花青素，以体积分数60%的甲醇为提取溶剂，在单因素试验基础上，运用Box-Behnken响应面法对液料比、超声时间、超声温度等关键工艺参数进行优化。最佳提取条件为：液料比70∶1 (mL∶g)、超声时间70 min、超声温度40 ℃，此条件下原花青素得率可达1.40%，工艺稳定可靠。采用优化工艺对16个乌饭树无性系叶片进行原花青素含量测定，结果显示不同无性系间含量变幅为2.41—128.36 mg/g，平均值为40.05 mg/g，变异系数高达96.7%。其中4号无性系含量最高（128.36 mg/g），是含量最低无性系11号（2.41 mg/g）的53.3倍。该研究结果为乌饭树高原花青素优异种质筛选及高值化开发利用提供了依据和技术支撑。
Abstract:
Using the leaves of Vaccinium bracteatum Thunb. as raw material, total proanthocyanidins were extracted by an ultrasonic-assisted method with 60% (v/v) methanol as the extraction solvent. On the basis of single-factor experiments, key process parameters including liquid-to-solid ratio, ultrasonic time, and ultrasonic temperature were optimized using Box-Behnken response surface methodology. The optimal extraction condition was determined as follows: liquid-to-solid ratio of 70∶1 (mL∶g), ultrasonic time of 70 min, and ultrasonic temperature of 40 ℃. Under this condition, the yield of proanthocyanidins could reach 1.40%, and the optimized process was stable and reliable. The validated method was subsequently applied to determine the proanthocyanidin content in leaves of 16 Vaccinium bracteatum clones collected from Jiangsu Academy of Forestry. The results revealed that the proanthocyanidin content among different clones ranged from 2.41 to 128.36 mg/g, with an average value of 40.05 mg/g with a coefficient of variation as high as 96.7%. Notably, Clone 4 showed the highest content (128.36 mg/g), which was 53.3 times as much as that of Clone 11 (2.41 mg/g), the lowest among the tested clones. These findings could provide scientific evidence and technical support for the screening of elite germplasm with high proanthocyanidin content and for the high-value development and utilization of Vaccinium bracteatum.

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备注/Memo

备注/Memo:
收稿日期：2025-12-21;修回日期：2026-01-09
基金项目：江苏省林业科学研究院自主科研项目“乌饭树原花青素生物合成途径关键酶基因的挖掘与解析”（ZZKY202202）
作者简介：于菲（2001- ），女，黑龙江鸡东人，硕士研究生。主要从事野生植物开发利用和组织培养技术研究。
*通信作者：张敏（1980- ），女，内蒙古乌海人，研究员，博士。主要从事生物技术与林木花卉良种繁育工作。
更新日期/Last Update: 2026-08-12