[1]沈艳,潘露,侯欢欢,等.植物病原真菌CFEM蛋白的理化性质及特征分析[J].江苏林业科技,2025,52(03):33-38.[doi:10.3969/j.issn.1001-7380.2025.03.006]
　Shen Yan,Pan Lu,Hou Huanhuan,et al.Analysis of physicochemical properties and characteristics of CFEM proteins in plant pathogenic fungi[J].Journal of Jiangsu Forestry Science &Technology,2025,52(03):33-38.[doi:10.3969/j.issn.1001-7380.2025.03.006]
点击复制

植物病原真菌CFEM蛋白的理化性质及特征分析()
分享到：

《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第52卷
期数:
2025年03期
页码:
33-38
栏目:
试验研究
出版日期:
2025-06-30

文章信息/Info

Title:
Analysis of physicochemical properties and characteristics of CFEM proteins in plant pathogenic fungi
文章编号:
1001-7380（2025）03-0033-06
作者:
沈艳1; 潘露2; 侯欢欢2; 祝友朋2*
1.大方县农业农村局，贵州 毕节 551700；
2.毕节市林业局，贵州 毕节 551700
Author(s):
Shen Yan1;  Pan Lu2;  Hou Huanhuan2;  Zhu Youpeng2*
1. Dafang County Agriculture and Rural Bureau, Bijie, Guizhou 551700, China;
2. Bijie Forestry Bureau, Bijie, Guizhou 551700, China
关键词:
植物病原真菌; CFEM蛋白; 理化性质; 生物信息学
Keywords:
Plant pathogenic fungus;  CFEM protein;  Physicochemical properties;  Bioinformatics
分类号:
S432.4；S763.15
DOI:
10.3969/j.issn.1001-7380.2025.03.006
文献标志码:
A
摘要:
CFEM蛋白是真菌中特有的一类蛋白，在真菌的生长发育和致病过程中发挥着重要作用。对前人研究发现的13个植物病原真菌的CFEM蛋白开展生物信息学分析，以期明确植物病原真菌CFEM理化性质及其特征。该研究通过Pfam数据库以“CFEM”为关键词搜索获得13个真菌的CFEM蛋白序列；通过SMART检查是否存在CFEM结构域，最终获得CFEM蛋白的序列，然后利用蛋白质数据库、Expasy等在线程序对蛋白序列的理化性质等特征进行分析。结果发现，CFEM蛋白主要为酸性和碱性蛋白、疏水性蛋白，亚细胞定位集中在质膜；二级结构组成特征为折叠较少，均具有CFEM保守结构域，分为5类，主要为CFEM、CFEM+TM，CFEM结构域序列氨基酸长度集中在60—66个，有7个间隔排列的半胱氨酸残基，具有高度的保守性。该文以期为解析植物病原真菌CFEM蛋白的功能和作用机制提供参考。
Abstract:
CFEM proteins are a special group of proteins in fungi, play a crucial role in fungal growth, development, and pathogenicity. This study conducted bioinformatics analysis based on 13 CFEM proteins from plant pathogenic fungi identified in previous research, aiming to clarify the physicochemical characteristics of these proteins in plant pathogenic fungi. We searched the Pfam database using the keyword “CFEM” and obtained the sequences of 13 fungal CFEM proteins. The presence of the CFEM domain was verified using SMART, and the sequences of the CFEM proteins were finally obtained. Subsequently, we analyzed the physicochemical properties of these protein sequences using online programs such as protein databases and Expasy. The results indicated that CFEM proteins are primarily acidic and basic proteins, as well as hydrophobic proteins. Their subcellular localization is primarily at the plasma membrane, and their secondary structure is characterized by fewer folds. All CFEM proteins possess a conserved CFEM domain, which can be classified into five categories, mainly CFEM and CFEM+TM. The amino acid length of the CFEM domain sequence ranges from 60 to 66, containing seven cysteine residues arranged at intervals, demonstrating a high degree of conservation. These findings provide valuable insights into the function and mechanism of action of CFEM proteins in plant pathogenic fungi.

参考文献/References:

[1]ZHANG Z N, WU Q Y, ZHANG G Z, et al. Systematic analyses reveal uniqueness and origin of the CFEM domain in fungi[J]. Scientific. Reports. 2015, 5: 13032.

[2]付秀霞,李志宇, 吴羽佳,等.植物病原真菌CFEM蛋白研究进展[J].福建农业学报,2022,37(12):1626-1632.
[3]KOU Y,TAN Y H,RAMANUJAM R,et al.Structure-function analyses of the Pth11 receptor reveal an important role for CFEM motif and redox regulation in rice blast[J].The New Phytologist,2017,214(1):330-342.
[4]GONG A D,JING Z Y,ZHANG K,et al. Bioinformatic analysis and functional characterization of the CFEM proteins in maize anthracnose fungus Colletotrichum graminicola[J].Journal of Integrative Agriculture,2020,19(2):541-550.
[5]井忠英,王国梁,刘文德.禾谷炭疽菌CFEM效应子的生物信息学鉴定与转录分析[J].植物保护,2016,42(1):81-86.
[6]张丽勍,段可,邹小花,等.草莓胶孢炭疽菌CFEM候选效应子的生物信息学鉴定及其侵染过程中的转录分析[J].植物保护,2017,43(5):43-51.
[7]刘思珍.辣椒胶孢炭疽菌两个CFEM效应蛋白的生物学功能研究[D].长沙:湖南大学,2021.
[8]WANG D,ZHANG D D,SONG J,et al.Verticillium dahliae CFEM proteins manipulate host immunity and differentially contribute to virulence[J]. BMC Biology, 2022, 20(1): 55.
[9]VAKNIN Y, SHADKCHAN Y, LEVDANSKY E,et al.The three Aspergillus fumigatus CFEM-domain GPI-anchored proteins (CfmA-C) affect cell-well stability but do not play a role in fungal virulence[J].Fungal Genetics and Biology,2014,63:55-64.
[10]LING J,ZENG F,GAO Y X,et al.Identifification of a class of CFEM proteins containing a new conserved motif in Fusarium oxyporum[J].Physiology and Molecular Plant Pathology,2015,89:41-48.
[11]ZHAO S,SHANG X,BI W,et al.Genome-wide identification of effector candidates with conserved motifs from the wheat leaf rust fungus Puccinia triticina[J]. Frontiers in Microbiology, 2020, 11:1188.
[12]QIAN Y L,ZHENG X Y,WANG X Y,et al. Systematic identification and functional characterization of the CFEM proteins in poplar fungus Marssonina brunnea[J].Frontiers in Cellular and Infection Microbiology,2022,12:1045615.
[13]陈玲巧.禾谷镰刀菌CFEM基因家族的生信分析及功能研究[D].上海:上海海洋大学,2022.

备注/Memo

备注/Memo:
收稿日期：2024-03-15;修回日期：2025-04-26
基金项目：国家级大学生创新创业训练计划项目（201710677013）
作者简介：沈艳（1996- ），女，云南曲靖人，硕士研究生，工程师。主要研究方向为农村发展。E-mail：1293541007@qq.com
*通信作者：祝友朋（1996- ），男，云南曲靖人，硕士研究生，工程师。主要研究方向为经济林木病害生物防治。E-mail：3420204485@qq.com
更新日期/Last Update: 2025-08-07