[1]袁雪,刘雪,解梦雨,等.枝龄、造伤及植物生长调节剂对牡丹‘富贵满堂’压条生根的影响[J].江苏林业科技,2025,52(03):1-7.[doi:10.3969/j.issn.1001-7380.2025.03.001]
　Yuan Xue,Liu Xue,Xie Mengyu,et al.Effect of age, wounding and plant growth regulators on tree peony variety ‘Fugui Mantang’ during layering[J].Journal of Jiangsu Forestry Science &Technology,2025,52(03):1-7.[doi:10.3969/j.issn.1001-7380.2025.03.001]
点击复制

枝龄、造伤及植物生长调节剂对牡丹‘富贵满堂’压条生根的影响()
分享到：

《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第52卷
期数:
2025年03期
页码:
1-7
栏目:
试验研究
出版日期:
2025-06-30

文章信息/Info

Title:
Effect of age, wounding and plant growth regulators on tree peony variety ‘Fugui Mantang’ during layering
文章编号:
1001-7380（2025）03-0001-07
作者:
袁雪1; 2; 刘雪3; 解梦雨1; 4; 袁涛1*
1.北京林业大学园林学院国家花卉工程技术研究中心，城乡生态环境北京实验室，林木花卉遗传育种教育部重点实验室，花卉种质创新与分子育种北京市重点实验室，北京100083；
2.黔西南民族职业技术学院，贵州 兴义 562400；
3.苏州市林业局相城分局，江苏 苏州 215100；
4.四川旅游学院，四川 成都 610100
Author(s):
Yuan Xue1; 2;  Liu Xue3;  Xie Mengyu1; 4;  Yuan Tao1*
1. School of Landscape Architecture，National Engineering Research Center for Floriculture，Beijing Laboratory of Urbanand Rural Ecological Environment，Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministryof Education，Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding，Beijing ForestryUniversity， Beijing 100083，China；
2. Southwest Guizhou Vocational and Technical College for Nationalities， Xingyi 562400，China；
3.Xiangcheng Branch of Suzhou Forestry Bureau，Suzhou 215100，China；
4.Sichuan Tourism University，Chengdu 610100，China
关键词:
牡丹; 压条; 环割; 横缢; 植物生长调节剂; 再生
Keywords:
Paeoni sect. Moutan Layering; Bark girdling; Strangulation; Plant growth regulator; Regeneration
分类号:
S685.11；S723.1+32.2
DOI:
10.3969/j.issn.1001-7380.2025.03.001
文献标志码:
A
摘要:
为探究牡丹压条繁殖过程中不同因素的影响，以北京市昌平区白浮村露地栽培的牡丹‘富贵满堂’为试验材料，在新枝抽生当年5—6月进行枝龄单因素压条试验，以及造伤方式（无、环剥、横缢）和植物生长调节剂种类（生根粉、IBA、NAA）及其质量浓度（0，500，1 200 mg/L）组合的L9(34)正交试验，统计生根情况、记录枝条形态，并对枝条的不定根、不定芽发生进行切片解剖结构观察。结果显示：（1）50 d枝龄的稍压条生根率高于80 d枝龄的压条生根率，但后者的新生根系质量较优；（2）造伤能促进生根，且环剥和横缢对不定根发生与生长的作用差异不显著；IBA促进生根的作用优于NAA和生根粉；生长调节剂最佳处理质量浓度是500 mg/L；（3）‘富贵满堂’不定根起源于维管形成层；（4）压条会在枝条覆土部位产生不定芽。在横缢伤口处涂抹500 mg/L IBA后进行压条繁殖，方法操作简便，能不产生愈伤组织直接诱导不定根和不定芽再生，该结果可为其他新优牡丹品种的高效繁殖提供参考。
Abstract:
To examine the effects of age, wounding, and hormones on peony organogenesis during layering, tree peony variety ‘Fugui Mantang’ plants, growing in Baifu Village, Changping District, Beijing, were used as the experimental material. Layering experiments were conducted in May and June after the new branches shot out. Results were showed that (1) The rooting rate of 50-day-old branches was slightly higher than that of 80-day-old branches, but the new root quality of the later was better. (2) L9 (34) orthogonal test demonstrated that wounding could promote the growth of roots after layering, and there was no significant difference on rooting rate and quality between bark girdling and strangulation. IBA, with ideal concentration of 500 mg/L, promoted rooting more effectively than NAA and rooting powder. (3) Anatomical structure observation revealed that the adventitious roots of ‘Fugui Mantang’ originated from vascular cambium. (4) Layering could result in adventitious buds at the soil-covered part of the branches. These adventitious buds might be related to the re-adjustment of hormones and nutrients in the mother plants, which could trigger the sprouting of active and dormant buds.The method of layering with strangulation and 500 mg/L IBA treatment is simple and easy to operate, which could directly regenerate adventitious roots and buds without callus. This article could serve as a hint for the highly efficient reproduction of other new superb peony varieties.

参考文献/References:

[1]曾端香,尹伟伦,赵孝庆,等.牡丹繁殖技术[J].北京林业大学学报,2000(3):90-95.

[2]尚文倩,王政,何松林,等.牡丹试管苗生根过程中内源IAA及相关酶活性的变化[J].西北农林科技大学学报(自然科学版),2021,49(2):129-136.
[3]张润龙,王小斌,邵灵梅,等.芍药和牡丹的组织培养及遗传转化体系构建[J].植物生理学报,2021,57(2):235-247.?/div>
[4]石宝锛.园林树木栽培学[M].北京:中国建筑工业出版社1999:118-120.
[5]RELF D, BALL E C. Propagation by cuttings, layering and division[M].Agricultural and Food Sciences,2009:1-6.
[6]HARTMANN H T, KESTER D E. Plant propagation:principles and practices[M].Ed 3d. Englewood Cliffs, NJ:Prentice-Hall,1975:880.
[7]解梦雨,徐珂,王心怡,等.黄化对牡丹枝条解剖结构及不定根发生的影响[J].四川农业大学学报,2022,40(6):901-906.
[8]ABDOU L, KARIM S, HABOU R, et al. Vegetative propagation trial of Prosopis africana (Guill. et Perr.) Taub. by air layering under Sudano-Sahelian climate in the South-Central Niger[J]. Journal of Botany, 2015 (2015):1-6.
[9]DAS A K,DAS S,PRASADB,et al.Assessment of rooting media for enhancing survivability and profitability of air layering in Litchi[J].International Journal of Agriculture Sciences,2016,8(51):23-26.
[10]刘盛免.果树环割环剥环扎技术[J].农家科技, 2010(8): 15.
[11]肖宏,于明革.环割、环剥和绞缢对甜樱桃幼树叶面积和成花的影响[J].韶关学院学报(自然科学版),2006(6):110-112.
[12]李丰.空中压条技术的改进及在茶树上的应用研究[D].泰安:山东农业大学, 2011.
[13]XIE M, JIANG Y M, XU K, et al. Strangulation and IBA treatments as an effective method to propagate tree peonies,Paeonia suffruticosa[J].Scientia Horticulturae,2023,307:111487.
[14]张帅,李荣生,尹光天,等.蛇皮果高空压条的促根措施[J].中南林业科技大学学报,2012,32(11):56-59.?/div>
[15]PUROHIT S, BHATT A, BHATT I D, et al. Propagation through air layering in Zanthoxylum armatum DC: an endangered medicinal plant in the himalayan region[J]. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences.2016,86:607-610.
[16]张海旺,王洪江,马艳丽,等.红醋栗压条繁殖效果研究[J].辽宁林业科技,2018(1):34-36.?/div>
[17]COLES Z S, DU TOIT E S. Open air-layering of Moringa oleifera utilizing seedling plug containers[J].South African Journal of Botany 2020,129:225-228.
[18]季孔庶,王章荣,陈天华,等.马尾松插穗生根能力变异的研究[J].南京林业大学学报,1998,22(3):66-70.?/div>
[19]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[20]廖维华,刘杰,石晓东,等.林木高空压条生根率影响因素分析[J].四川林业科技,2018,39(2):103-107.
[21]许建成,惠振彪,孙婧瑜,等.榆林沙地中条欧李压条繁殖试验[J].南方农业,2019,13(29):43-44.?/div>
[22]温吉华,高坤金,徐焕录,等.幼旺李树环剥、环割、绞缢试验[J].河北果树,2002(2): 9-10.
[23]MOORE G M. Ring-barking and girdling: how much vascular connection do you need between roots and crown? [C]//The 14th National Street Tree Symposium,Melbourne,2013:87-96.
[24]杨利粉,孟红志,马宏,等.绞缢对苹果矮砧压条新梢激素含量及生根相关基因表达的影响[J].园艺学报,2017,44(4):613-621.?/div>
[25]许智宏,张宪省,苏英华,等.植物细胞全能性和再生[J].中国科学:生命科学,2019,49(10):1282-1300.
[26]BARTEL B, LECLERE S, MAGIDIN M, et al. Inputs to the active indole-3-acetic acid pool: de novo synthesis, conjugate hydrolysis, and indole-3-butyric acid β-oxidation [J].Journal of Plant Growth Regulation,2001,20(3):198-216.
[27]朱晓宇,童婉婉,赵楚,等.冬青‘长叶阿尔塔’扦插生根及解剖学研究[J].浙江农林大学学报,2022,39(2):347–355.
[28]蒋运生,韦霄,漆小雪,等.金花茶高空压条繁殖技术[J].福建林业科技,2010,37(1):68-71.
[29]郑雨盼,杨锦昌,邹文涛,等.常用促根生长调节剂对闽楠高空压条生根的影响[J].热带作物学报,2020,41(9):1803-1807.
[30]王莲英.中国牡丹品种图志[M].北京:中国林业出版社,1997.
[31]朱耀军.牡丹茎扦插繁殖技术及生根机理初步研究[D].北京:中国林业科学研究院,2007.?/div>
[32]徐麟,吴双.植物再生的理论发展和应用革新[J].植物生理学报,2020,56(8):1651-1652.
[33]CHEN X, CHENG J, CHEN L, et al. Auxin-independent NAC pathway acts in response to explant-specific wounding and promotes root tip emergence during de novo root organogenesis in Arabidopsis[J].Plant Physiology,2016,170 (4):2136–2145.
[34]SU Y H, ZHANG X S.The hormonal control of regeneration in plants[J].Mechanism of Regeneration,2014,108:35-69.
[35]张焕欣,董春娟,李福凯,等.植物不定根发生机理的研究进展[J].西北植物学报,2017,37(7):1457-1464.

相似文献/References:

[1]祝亚云,孙海楠,蒋泽平*,等.‘凤丹’单株间生长及结实性状的变异分析[J].江苏林业科技,2017,44(06):14.[doi:10.3969/j.issn.1001-7380.2017.06.004]
　ZHU Ya-yun,SUN Hai-nan,JIANG Ze-ping*,et al.Analysis on variation of growth and reproductive traits of oil peony ‘Fengdan’[J].Journal of Jiangsu Forestry Science &Technology,2017,44(03):14.[doi:10.3969/j.issn.1001-7380.2017.06.004]
[2]周洁,黄婧,孙体如,等.牡丹花型基因的研究[J].江苏林业科技,2020,47(02):6.[doi:10.3969/j.issn.1001-7380.2020.02.002]
　Zhou Jie,Huang Jing,Sun Tiru,et al.Flowering type genes study of peonies based on transcriptome comparison[J].Journal of Jiangsu Forestry Science &Technology,2020,47(03):6.[doi:10.3969/j.issn.1001-7380.2020.02.002]

备注/Memo

备注/Memo:
收稿日期：2024-04-21;修回日期：2025-05-07
基金项目：国家林业和草原局行业标准项目“牡丹综合体”（2018-LY-054）；北京林业大学建设世界一流学科和特色发展引导专项（2019XKJS0324）
作者简介：袁雪(1994- )，女，贵州兴义人，博士在读。主要从事花卉种质资源与遗传育种研究。E-mail: yuanxue2022@bjfu.edu.cn
*通信作者：袁涛(1969- )，四川夹江人，教授，主要从事花卉种质资源与遗传育种、繁殖与栽培研究。E-mail:yuantao@bjfu.edu.cn
更新日期/Last Update: 2025-08-07