[1]周 鹏,张 敏*,吴双竹,等.不同红蓝光质比LED光源处理对铁皮石斛试管苗移栽的影响[J].江苏林业科技,2017,44(03):19-22.[doi:10.3969/j.issn.1001-7380.2017.03.005]
　ZHOU Peng,ZHANG Min*,WU Shuang-zhu,et al.Effect of different light quality ratios of light-emitting diodes on Dendrobium officinal transplant growth from in vitro culture[J].Journal of Jiangsu Forestry Science &Technology,2017,44(03):19-22.[doi:10.3969/j.issn.1001-7380.2017.03.005]
点击复制

不同红蓝光质比LED光源处理对铁皮石斛试管苗移栽的影响()
分享到：

《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第44卷
期数:
2017年03期
页码:
19-22
栏目:
试验研究
出版日期:
2017-06-30

文章信息/Info

Title:
Effect of different light quality ratios of light-emitting diodes on Dendrobium officinal transplant growth from in vitro culture
文章编号:
1001-7380（2017）03-0019-04
作者:
周 鹏; 张 敏*; 吴双竹; 黄 婧
江苏省林业科学研究院，江苏 南京 211153
Author(s):
ZHOU Peng;  ZHANG Min*;  WU Shuang-zhu;  HUANG Jing
Jiangsu Academy of Forestry, Nanjing 211153, China
关键词:
铁皮石斛; LED; 离体培养; 移栽
Keywords:
Dendrobium officinal;  LED;  In vitro culture;  Transplant
分类号:
Q943.1；S567.23+.9
DOI:
10.3969/j.issn.1001-7380.2017.03.005
文献标志码:
A
摘要:
采用不同红蓝光质比(R, R7B3, R5B5, R3B7, B)的LED为光源，研究不同光源对铁皮石斛试管苗移栽后生长的影响，探讨适合铁皮石斛试管苗移栽的光质条件。结果表明：与对照荧光灯相比，LED红蓝复合光能提高铁皮石斛移栽成活率，其中，R5B5和R7B3下成活率显著高于其他处理（P＜0.05）。红光有利于铁皮石斛茎段和叶片的伸长，但不利于试管苗移栽成活；红蓝复合光有利于移栽苗叶片数增加，且促进移栽苗生根及根系伸长。在生物量积累方面，R7B3处理下铁皮石斛移栽苗鲜质量和干质量均达到最大值。综合分析，R7B3是适宜铁皮石斛试管苗移栽的最佳光质比。该研究结果为LED光源在铁皮石斛组织培养中的应用奠定了基础。
Abstract:
In order to discuss the best light quality for transplanted Dendrobium officinal in vitro, the effects of light-emitting diodes and the cold cathode fluorescent lamp in the production for transplanted D. officinal in vitro were studied. The results showed that the mixture of red and blue LEDs had a positive effect on survival rate. And the survival rate in the LED of R5B3 and R7B3 was significantly higher than others (P＜0.05)．Under red light illumination, plant height and leaf length were promoted, but survival rate was reduced. Number of leaves, roots and the root length were increased in the mixture of red and blue LEDs. The fresh weight and dry weight all reached the maximum in the LED of R7B3. To sum up, the LED of R7B3 was suitable for the transplanted D. officinal in vitro. The present study provides a proof of application of LEDs for transplanted D. officinal in vitro.

参考文献/References:

［1］聂少平, 蔡海兰. 铁皮石斛活性成分及其功能研究进展[J]. 食品科学, 2012, 33(23):356-361.
［2］陈晓梅, 王春兰, 杨峻山,等. 铁皮石斛化学成分及其分析的研究进展[J]. 中国药学杂志, 2013, 48(19):1634-1640.
［3］赵兴兵, 吴维佳, 庞 璐,等. 珍稀濒危药材铁皮石斛组培快繁关键技术研究[J]. 湖南中医药大学学报, 2012, 32(3):27-30.
［4］许奕, 宋顺, 王安邦,等. 不同培养基对铁皮石斛壮苗生根的影响及移栽条件优化[J]. 江苏农业科学, 2015, 43(8):247-249.
［5］严旭超, 钟均宏, 周颖军,等. 铁皮石斛试管苗移栽与大棚栽培管理[J]. 园艺与种苗, 2015(11):51-52.
［6］吴雅, 史骥清, 滕士元,等. 铁皮石斛组培苗移栽基质的筛选[J]. 现代农业科技, 2010, 2010(6):107-108.
［7］GUPTA S D, JATOTHU B. Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis[J]. Plant Biotechnology Reports, 2013, 7(3):211-220.
［8］任桂萍, 王小菁, 朱根发. 不同光质的LED对蝴蝶兰组织培养增殖及生根的影响[J]. 植物学报, 2016, 51(1):81-88.
［9］MA X, WANG Y, LIU M, et al. Effects of green and red lights on the growth and morphogenesis of potato ( Solanum tuberosum L.) plantlets in vitro[J]. Scientia Horticulturae, 2015, 190:104-109.
［10］CAO D H, HONG C H, KIM S K, et al. LED light for in vitro and ex vitro efficient growth of economically important highbush blueberry ( Vaccinium corymbosum L.)[J]. Acta Physiologiae Plantarum, 2016, 38(6):1-9.
［11］杨维杰, 梁 君, 杨林林. 不同LED光质对铁皮石斛生长及多糖含量的影响[J]. 浙江农业科学, 2015, 56(8):1188-1190.
［12］尚文倩, 王 政, 侯甲男, 等. 不同红蓝光质比LED光源对铁皮石斛试管苗生长的影响[J]. 西北农林科技大学学报(自然科学版), 2013, 41(5):155-159.
［13］陈星星, 徐明辉, 何松林. 新型光源发光二极管(LED)下白掌组培苗移栽后生长状况研究[J]. 中国农学通报, 2014，30(19):196-200.
［14］CAO D H, HONG C H, JUNG H B, et al. Growth and morphogenesis of encapsulated strawberry shoot tips under mixed LEDs[J]. Scientia Horticulturae, 2015, 194:194-200.
［15］吴 雅, 史骥清, 滕士元,等. 铁皮石斛组培苗移栽基质的筛选[J]. 现代农业科技, 2010(6):107-108.
［16］魏 星, 顾 清, 戴艳娇,等. 不同光质对菊花组培苗生长的影响[J]. 中国农学通报, 2008, 24(12):344-349.

相似文献/References:

[1]周鹏,张敏*.LED光质对植物组织培养影响研究进展[J].江苏林业科技,2016,43(04):44.[doi:10.3969/j.issn.1001-7380.2016.04.012]
　ZHOU Peng,ZHANG Min*.Application of light-emitting diodes (LEDs) in in vitro plant tissue culture: A review[J].Journal of Jiangsu Forestry Science &Technology,2016,43(03):44.[doi:10.3969/j.issn.1001-7380.2016.04.012]
[2]余水生,刘菊莲,王怡娟,等.梵净山石斛与铁皮石斛矿质元素及有效成分比较[J].江苏林业科技,2018,45(01):32.[doi:10.3969/j.issn.1001-7380.2018.01.008]
　YU Shui-sheng,LIU Ju-lian,WANG Yi-juan,et al.Comparison of mineral elements and active ingredients of Dendrobium fanjingshanense and Dendrobium officinale[J].Journal of Jiangsu Forestry Science &Technology,2018,45(03):32.[doi:10.3969/j.issn.1001-7380.2018.01.008]
[3]黄婧,周鹏,隋德宗,等.不同LED光质对构树组织培养苗生物量和营养物质含量的影响[J].江苏林业科技,2020,47(03):22.[doi:10.3969/j.issn.1001-7380.2020.03.005]
　Huang Jing,Zhou Peng,Sui Dezong,et al.Effect of different LED lights on the biomass accumulation and nutriment content of Broussonetia papyrifera tissue culture[J].Journal of Jiangsu Forestry Science &Technology,2020,47(03):22.[doi:10.3969/j.issn.1001-7380.2020.03.005]
[4]黄婧,周鹏,李飞,等.LED光质对乌饭树幼苗生长及叶片营养物质含量的影响[J].江苏林业科技,2023,50(01):7.[doi:10.3969/j.issn.1001-7380.2023.01.002]
　Huang Jing,Zhou Peng,Li Fei,et al.Effects of LED light sources on growth and nutrient contents in leaves of Vaccinium bracteatum seedlings[J].Journal of Jiangsu Forestry Science &Technology,2023,50(03):7.[doi:10.3969/j.issn.1001-7380.2023.01.002]
[5]黄婧,周鹏,李飞,等.LED光质对乌饭树叶片叶绿素荧光参数的影响[J].江苏林业科技,2023,50(03):1.[doi:10.3969/j.issn.1001-7380.2023.03.001]
　Huang Jing,Zhou Peng,Li Fei,et al.Effect of different LED light quality on chlorophyll fluorescence parameters of Vaccinium bracteatum leaves[J].Journal of Jiangsu Forestry Science &Technology,2023,50(03):1.[doi:10.3969/j.issn.1001-7380.2023.03.001]

备注/Memo

备注/Memo:
收稿日期：2017-06-04;修回日期：2017-06-20
基金项目：江苏省林业三新工程项目“名贵中药铁皮石斛高效栽培技术集成与示范推广”（LYSX[2015]27）
作者简介：周 鹏（1989- ），男，江苏东台人，研究实习员，硕士。主要从事树木资源学和树木生理生化研究。
*通信作者：张 敏（1980- ），女，内蒙古人，副研究员，博士。主要从事生物技术与林木花卉良种繁育工作。
更新日期/Last Update: 2017-07-19