[1]周鹏,张敏*.LED光质对植物组织培养影响研究进展[J].江苏林业科技,2016,43(04):44-48.[doi:10.3969/j.issn.1001-7380.2016.04.012]
 ZHOU Peng,ZHANG Min*.Application of light-emitting diodes (LEDs) in in vitro plant tissue culture: A review[J].Journal of Jiangsu Forestry Science &Technology,2016,43(04):44-48.[doi:10.3969/j.issn.1001-7380.2016.04.012]
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LED光质对植物组织培养影响研究进展()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第43卷
期数:
2016年04期
页码:
44-48
栏目:
综述与专论
出版日期:
2016-08-30

文章信息/Info

Title:
Application of light-emitting diodes (LEDs) in in vitro plant tissue culture: A review
文章编号:
1001-7380(2016)04-0044-05
作者:
周鹏张敏*
江苏省林业科学研究院,江苏 南京211153
Author(s):
ZHOU Peng ZHANG Min*
Jiangsu Academy of Forestry, Nanjing 211153, China
关键词:
LED植物组织培养光质愈伤组织分化增殖
Keywords:
Light-emitting diodePlant tissue cultureLight qualityCallusDifferentiationMultiplication
分类号:
Q943.1;O432.3
DOI:
10.3969/j.issn.1001-7380.2016.04.012
文献标志码:
A
摘要:
发光二极管(LED)作为一种比传统光源更为有效的替代光源,越来越多地被应用于植物组织培养中。近年来学者围绕 LED光源在植物组织培养中的应用进行了大量的研究,并已证明植物组织培养过程的各个阶段均受到LED光质的调控。该文就LED光质调控植物组织培养的研究现状进行概述,重点介绍了LED光质对植物愈伤组织的诱导和分化、组织培养苗增殖、生长发育和生根壮苗等方面的影响,并对今后LED光质调控的研究进行了展望。
Abstract:
As a new and effective lighting systems, light-emitting diodes (LEDs) has been extensively used in plant tissue culture.To investigate effects of LEDs on plants, lots of studies have been conducted, which has proved that various stages of plants grown in vitro have been regulated by light quality of LEDs. This article gives an review of the effects of light quality of LEDs on the growth and differentiation of plant callus, seedlings multiplication, the growth and development of plants and rooting in vitro, and also describes the prospects of the study on light quality of LED in the future.

参考文献/References:

[1]VIEIRA L D N, FRAGA H P D F, ANJOS K G D, et al. Light-emitting diodes (LED) increase the stomata formation and chlorophyll content in Musa acuminata (AAA) ‘Nanicao Corupá’ in vitro plantlets[J]. Theoretical & Experimental Plant Physiology, 2015, 27(2):91-98.
[2]SHIN K S, MURTHY H N, HEO J W, et al. The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants[J]. Acta Physiologiae Plantarum, 2008,30(3):339-343.
[3]刘浩, 李胜, 马绍英, 等. LED不同光质对萝卜愈伤组织诱导、增殖和萝卜硫素含量的影响[J]. 植物生理学通讯, 2010(4):347-350.
[4]BUDIARTO K. Spectral quality affects morphogenesis onAnthurium plantlet during in vitro culture [J]. Agrivita Journal of Agricultural Science, 2010, 32(3):234-240.
[5]黄丽华, 洪亚辉, 戴雄泽, 等. 光质对辣椒离体愈伤组织诱导及分化的影响[J]. 湖南农业大学学报(自然科学版), 2009,35(6):615-617.
[6]马琳, 刘世琦, 张自坤, 等. 光质对大蒜愈伤组织诱导、增殖及器官分化的影响[J]. 西北农业学报, 2011(06):118-122.
[7]NHUT D T, HUY N P, TAI N T, et al. Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Panax vietnamensis Ha et Grushv. [J]. Biotechnology & Biotechnological Equipment, 2015,29(2):299-308.
[8]薛冲, 李胜, 马绍英, 等. 不同光质对西兰花愈伤组织及萝卜硫素含量的影响[J]. 甘肃农业大学学报, 2010,45(4):95-99.
[9]刘媛, 李胜, 马绍英, 等. 肉桂酸和不同光质对葡萄愈伤组织增殖及白藜芦醇累积的效应[J]. 甘肃农业大学学报, 2010,45(1):41-46.
[10]郭君丽, 陈明霞, 李明军, 等. 光质和生长物质组合对怀山药零余子脱分化和再分化的影响[J]. 河南师范大学学报(自然科学版), 2003,31(2):99-102.
[11]刘媛, 李胜, 马绍英, 等. 不同光质对葡萄试管苗离体培养生长发育的影响[J]. 园艺学报, 2009,36(8):1105-1112.
[12]任桂萍, 王小菁, 朱根发. 不同光质的LED对蝴蝶兰组织培养增殖及生根的影响[J]. 植物学报, 2016,51(1):81-88.
[13]李 黎, 陈 菲, 唐焕伟, 等. LED不同光质对菊花组培苗的影响[J]. 国土与自然资源研究, 2015(4):82-83.
[14]王玉英, 丁绪杰, 刘昳雯. LED不同光质对烟草组培苗生长发育的影响[J]. 安徽农业科学, 2015(17):38-39.
[15]杨长娟, 凌 青, 任兴平, 等. LED不同光质对洋桔梗组培苗增殖的影响[J]. 北方园艺, 2011(18):154-156.
[16]樊丽娜, 何慧怡, 曾巧英, 等. LED不同光质对甘蔗愈伤组织分化及增殖的影响[J]. 甘蔗糖业, 2014(4):1-5.
[17]陈 志, 孙庆丽, 汪一婷, 等. 不同光质对甘蔗组培苗的影响[J]. 农业工程, 2012,2(10):51-57.
[18]刘晓青, 苏家乐, 陈尚平, 等. 高山杜鹃叶片再生和试管苗生长对不同LED光质的响应特征[J]. 江苏农业学报, 2013,29(6):1451-1455.
[19] 张 欢, 徐志刚, 崔 瑾, 等. 不同光谱能量分布对菊花试管苗增殖及生根的影响[J]. 园艺学报, 2010,37(10):1629-1636.
[20] 尚文倩, 王 政, 侯甲男,等. 不同红蓝光质比LED光源对铁皮石斛试管苗生长的影响[J]. 西北农林科技大学学报(自然科学版), 2013,41(5):155-159.
[21] 陈星星, 邵秀丽, 何松林. LED光源不同光质比对白掌组培苗生长的影响[J]. 北方园艺, 2015(6):86-89.
[22] HAHN E J, KOZAI T, PAEK K Y. Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro growth of Rehmannia glutinosa plantlets[J]. Journal of Plant Biology, 2000, 43(4):247-250.
[23]KIM S J, HAHN E J, HEO J W, et al. Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro[J]. Scientia Horticulturae, 2004, 101(1-2):143-151.
[24]LI H, XU Z, TANG C. Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutumL.) plantlets in vitro[J]. Plant Cell, Tissue & Organ Culture, 2010, 103(2):155-163.
[25]HEO J W, KONG S S, KIM S K, et al. Light quality affects in vitro growth of grape ‘Teleki 5BB’[J]. Journal of Plant Biology, 2006, 49(4):276-280.
[26]NHUT D T, TAKAMURA T, WATANABE H, et al. Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs)[J]. Plant Cell, Tissue & Organ Culture, 2003, 73(1):43-52.
[27]陈颖, 王政, 纪思羽, 等. LED光源不同光质比例对红掌试管苗生长的影响[J]. 江西农业大学学报, 2013,35(2):375-380.
[28]NHUT D T, NHUT D T, DON N T, et al. Light-emitting diodes as an effective lighting source for in vitro banana culture[M]// JAIN M S, HGGMAN H. Protocols for Micropropagation of Woody Trees and Fruits. Springer Netherlands, 2007:527-541.
[29]LUAN V Q, HUY N P, NAM N B, et al. Ex vitro and in vitro Paphiopedilum delenatii Guillaumin stem elongation under light-emitting diodes and shoot regeneration via stem node culture[J]. Acta Physiologiae Plantarum, 2015, 37(7):1-11.
[30]POUDEL P R, KATAOKA I, MOCHIOKA R. Effect of red- and blue-light-emitting diodes on growth and morphogenesis of grapes[J]. Plant Cell, Tissue & Organ Culture, 2007, 92(2):147-153.
[31]HEO J, LEE C, CHAKRABARTY D, et al. Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED)[J]. Plant Growth Regulation, 2002, 38(3):225-230.
[32]MOON H K, PARK S Y, YONG W K, et al. Growth of Tsuru-rindo (Tripterospermum japonicum ) cultured in vitro, under various sources of light-emitting diode (LED) irradiation[J]. Journal of Plant Biology, 2006, 49(2):174-179. [J].
[33]WANG X Y, XU X M, CUI J. The importance of blue light for leaf area expansion, development of photosynthetic apparatus, and chloroplast ultrastructure of Cucumis sativus grown under weak light[J]. Photosynthetica, 2015, 53(2):213-222.
[34]LIN Y, LI J, LI B, et al. Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro[J]. Plant Cell, Tissue & Organ Culture, 2011, 105(3):329-335.
[35]FAN X X, JIE Z, XU Z G, et al. Effects of different light quality on growth, chlorophyll concentration and chlorophyll biosynthesis precursors of non-heading Chinese cabbage (Brassica campestris L.)[J]. Acta Physiologiae Plantarum, 2013, 35(9):2721-2726.
[36]魏星, 顾清, 戴艳娇, 等. 不同光质对菊花组培苗生长的影响[J]. 中国农学通报, 2008,24(12):344-349.
[37]GUPTA S D, JATOTHU B. Fundamentals and applications of light-emitting diodes (LEDs) inin vitro plant growth and morphogenesis[J]. Plant Biotechnology Reports, 2013, 7(3):211-220.
[38]WU H C, TOIT E S D. In vitro organogenesis of Protea cynaroides L. shoot-buds cultured under red and blue light-emitting diodes[M] // Embryogenesis. Springer Netherlands,2012:151-166.
[39]KUPILCIK A, CANOVA R M, DAPKUNIEN E S, et al. In vitro culture of Chrysanthemum plantlets using light-emitting diodes[J]. Central European Journal of Biology, 2008, 3(3):161-167.
[40]邸秀茹, 焦学磊, 崔 瑾, 等. 新型光源LED辐射的不同光质配比光对菊花组培苗生长的影响[J]. 植物生理学通讯, 2008,44(4):661-664.
[41]ALVARENGA I C A, PACHECO F V, SILVA S T, et al. In vitro culture of Achillea millefolium L.: quality and intensity of light on growth and production of volatiles[J]. Plant Cell, Tissue & Organ Culture, 2015, 122(2):299-308.
[42]WANG J, LU W, TONG Y, et al. Leaf morphology, photosynthetic performance, chlorophyll fluorescence, stomatal development of lettuce (Lactuca sativa L.) exposed to different ratios of red light to blue light[J]. Frontiers in Plant Science, 2016(7):250.
[43]NHUT D T, TAKAMURA T, WATANABE H, et al. Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs)[J]. Plant Cell, Tissue & Organ Culture, 2003, 73(1):43-52.
[44]NHUT D T, TAKAMURA T, WATANABE H, et al. Artificial light source using light-emitting diodes (LEDs) in the efficient micropropagation of Spathiphyllum plantlets[J]. Acta Horticulturae, 2005, 692(692):137-142.

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备注/Memo

备注/Memo:
收稿日期:2016-07-29;修回日期:2016-08-08
基金项目:江苏省农业三新工程项目“金叶络石规模化繁育与示范推广”(SXGC[2015]296)
作者简介:周鹏(1989- ),男,助理研究员,硕士,主要从事植物组织培养技术研究。
*通信作者:张敏(1980- ),女,副研究员,博士,主要从事生物技术与林木花卉良种繁育工作。
更新日期/Last Update: 2016-09-08