[1]李燕,黄婧,张启香,等.农杆菌介导微型月季遗传转化中抗生素的优化[J].江苏林业科技,2025,52(02):1-9.[doi:10.3969/j.issn.1001-7380.2025.02.001]
　Li Yan,Huang Jing,Zhang Qixiang,et al.Optimization of antibiotics and culture conditions in Agrobacterium-mediated infection of miniature roses[J].Journal of Jiangsu Forestry Science &Technology,2025,52(02):1-9.[doi:10.3969/j.issn.1001-7380.2025.02.001]
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农杆菌介导微型月季遗传转化中抗生素的优化

《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第52卷
期数:
2025年02期
页码:
1-9
栏目:
试验研究
出版日期:
2025-04-30

文章信息/Info

Title:
Optimization of antibiotics and culture conditions in Agrobacterium-mediated infection of miniature roses
文章编号:
1001-7380（2025）02-0001-09
作者:
李燕1; 黄婧2; 张启香1; 张敏2*
1. 浙江农林大学，浙江 杭州 311300；
2. 江苏省林业科学研究院，江苏 南京 211153
Author(s):
Li Yan1; Huang Jing2; Zhang Qixiang1; Zhang Min2*
1. Zhejiang A&F University，Hangzhou 311300，China；
2. Jiangsu Academy of Forestry，Nanjing 211153，China
关键词:
微型月季; 遗传转化; 抗生素; 培养; 农杆菌
Keywords:
Miniature rose; Genetic transformation; Antibiotic; Tissue culture; Agrobacterium
分类号:
Q943.1；Q943.2；Q944.6
DOI:
10.3969/j.issn.1001-7380.2025.02.001
文献标志码:
A
摘要:
通过对3种抗生素的筛选，确立最适抑制农杆菌过度生长、促进微型月季愈伤组织生长与分化的抗生素及其浓度，从而建立稳定而有效的微型月季遗传转化体系。结果表明：微型月季叶片培养于150 mg/L头孢噻肟钠培养基可抑制农杆菌生长且利于材料生长分化。侵染最佳条件为：侵染菌液OD600为0.5—0.6，侵染5—10 min，共培养2 d。‘荷仙姑’‘彩虹’和‘笑脸’微型月季阳性愈伤的PCR阳性率分别为26.4%，18.4%和31.2%。该研究结果为微型月季农杆菌遗传转化的抗生素选择和培育条件的优化提供一定参考。
Abstract:
By using three antibiotics (cefotaxime sodium，carbenicillin and ampicillin)，the most suitable antibiotics and their concentrations for inhibiting excessive growth of Agrobacterium and promoting the growth and differentiation of miniature rose leaf callus were determined, thus to establish a stable and effective genetic transformation system for miniature roses. The results indicated that the transforming leaves cultured in 150 mg/L cefotaxime sodium medium could inhibit the growth of Agrobacterium and promote callus growth and differentiation. The optimal conditions for infection were as follows: an OD600 value of 0.5—0.6 in the infection solution, infection duration of 5—10 min, and co-cultivation for 2 days. The PCR positive transformants rates of ‘Hexiangu’ ‘Rainbow’ and ‘Smiling Face’ miniature rose positive callus were 26.4%, 18.4%, and 31.2%, respectively. The results could provide some reference for the selection of antibiotics and optimization of cultivation conditions in the genetic transformation of Agrobacterium.

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备注/Memo

备注/Memo:
收稿日期：2024-12-24;修回日期：2025-01-19
基金项目：江苏省林业科学研究院自主科研项目“无（稀）皮刺月季新品种选育”（ZZKY202302）；江苏省省级作物种质圃（月季）保种项目（2024-SJ-021）；江苏省科技资源（农业种质）统筹服务平台月季种质资源圃
作者简介：李燕（1999- ），女，云南昆明人，研究生。主要从事林业生物技术研究。
*通信作者：张敏（1980- ），女，内蒙古乌海人，研究员，博士。主要从事生物技术与林木花卉良种繁育工作。
更新日期/Last Update: 2025-06-16