[1]王锐,李小林,张艾琳,等.毛竹JAZ基因家族的鉴定与表达分析[J].江苏林业科技,2023,50(06):1-9.[doi:10.3969/j.issn.1001-7380.2023.06.001]
　Wang Rui,Li Xiaolin,Zhang Ailin,et al.Genome-wide identification and expression ofthe JAZ gene family in Phyllostachys edulis[J].Journal of Jiangsu Forestry Science &Technology,2023,50(06):1-9.[doi:10.3969/j.issn.1001-7380.2023.06.001]
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毛竹JAZ基因家族的鉴定与表达分析()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第50卷
期数:
2023年06期
页码:
1-9
栏目:
试验研究
出版日期:
2023-12-31

文章信息/Info

Title:
Genome-wide identification and expression ofthe JAZ gene family in Phyllostachys edulis
文章编号:
1001-7380（2023）06-0001-09
作者:
王锐1; 李小林2; 张艾琳3; 毕凌凤3; 吴彩云3; 黄从德3; 陈刚3*
1.四川省林业科学研究院,四川 成都 610081;
2. 四川省林业工作总站,四川 成都 610084;
3. 四川农业大学林学院, 四川 成都 611130
Author(s):
Wang Rui1;  Li Xiaolin2;  Zhang Ailin3;  Bi Lingfeng3; ?u Caiyun3;  Huang Congde3;  Chen Gang3*
1 .Sichuan Academy of Forestry ,Chengdu 610081 ,China；
2.Forestry Station of Sichuan Province,Chengdu 610084,China;?/div>
3.College of Forestry, Sichuan Agricultural University,Chengdu 611130,China
关键词:
毛竹; JAZ基因家族; 基因表达; 植物防御
Keywords:
Phyllostachys edulis;  JAZ gene family;  Gene expression;  Plant defense
分类号:
Q943.2；S795.7
DOI:
10.3969/j.issn.1001-7380.2023.06.001
文献标志码:
A
摘要:
茉莉酸（jasmonic acid, JA）作为重要植物生长调节剂之一，调控植物抵抗各类胁迫，其中，JAZ（jasmonate ZIM-domain）作为茉莉酸通路的抑制因子，调控JA与其他激素信号的交互反应。为了探究毛竹（Phyllostachys edulis）中JAZ基因与其他植物调节剂的交互作用以及在不同组织中的表达，该研究通过隐马尔科夫模型（HMM）对毛竹全基因组搜索，共鉴定出37条JAZ基因并进行定位，分析了该家族基因编码蛋白质的理化性质、系统进化、共线性关系及其在不同组织和激素处理下的表达模式。研究结果表明：该37条JAZ基因归属于4个不同亚族，亚族之间基序和结构域变异较大。共线性分析揭示毛竹JAZ家族在演化过程中存在全基因组多倍化事件，与另一单子叶植物水稻（Oryza sativa）在进化上较近。萘乙酸（NAA）促进了大部分JAZ基因的表达，但赤霉素（GA）处理存在较大异质性，另外，JAZ基因主要在毛竹叶片中表达，在竹鞭中基本不表达。以上结果为毛竹JAZ基因功能鉴定提供了重要参考，对于毛竹生长和防御的分子机制研究打下了基础。
Abstract:
Jasmonic acid is one of the important plant endogenous hormones, regulating plant defense against various biological and abiotic stresses. JAZ (jasmonate ZIM-domain) is an inhibitor of jasmonic acid pathway, and plays an important role in the interaction between JA signal and other hormone signals. In this study, the Hidden Markov Model (HMM) was used to search the genome of Phyllostachys edulis. A total of 37 JAZ genes were identified and localized, and the physicochemical properties, phylogeny, collinearity and expression of the proteins encoded by the genes in this family were analyzed. The results showed that the 37 JAZ genes belonged to 4 different subfamilies (Group A-D), and there were great variations in the motif and domain among the subfamilies. Collinearity analysis revealed that the JAZ family of bamboo has a genome-wide polyploidy event during its evolution, which is relatively close to another monocotyledonous plant, rice (Oryza sativa). NAA promoted the expression of most JAZ genes, but the effect of GA treatment was quite different. In addition, JAZ genes were mainly expressed in the leaves of bamboo, but not in the rhizome. These results provide an important reference for the identification of JAZ gene function of bamboo, and lay a foundation for the study of molecular mechanism of growth and defense of bamboo.

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备注/Memo

备注/Memo:
收稿日期：2023-09-16;修回日期：2023-10-26
基金项目：四川省“十四五”农作物及畜禽育种攻关项目“笋用竹种资源创制与配套技术研究”（2021YFYZ0006）
作者简介：王锐(1989- ),男,四川南充人,工程师。研究方向为经济林栽培学。
*通信作者：陈刚(1989- ),男,四川巴中人,副教授,硕士生导师。研究方向为林木栽培生理与森林生态。
更新日期/Last Update: 2024-03-14