[1]冯景,邓衍明*,齐香玉,等.圆锥绣球‘石灰灯’种子形态及萌发特性研究[J].江苏林业科技,2022,49(03):9-14.[doi:10.3969/j.issn.1001-7380.2022.03.002]
 Feng Jing,Deng Yanming*,Qi Xiangyu,et al.Studies on the seed morphology and germination characteristics of Hydrangea paniculata ‘Limelight’[J].Journal of Jiangsu Forestry Science &Technology,2022,49(03):9-14.[doi:10.3969/j.issn.1001-7380.2022.03.002]
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圆锥绣球‘石灰灯’种子形态及萌发特性研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第49卷
期数:
2022年03期
页码:
9-14
栏目:
试验研究
出版日期:
2022-06-30

文章信息/Info

Title:
Studies on the seed morphology and germination characteristics of Hydrangea paniculata ‘Limelight’
文章编号:
1001-7380(2022)03-0009-06
作者:
冯景邓衍明*齐香玉陈双双陈慧杰刘廷利
江苏省农业科学院休闲农业研究所/江苏省高效园艺作物遗传改良重点实验室,江苏 南京 210014
Author(s):
Feng JingDeng Yanming*Qi XiangyuChen ShuangshuangChen HuijieLiu Tingli
Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences/ Jiangsu Provincial Key Laboratory forthe Genetics and Improvement of Horticultural Crops, Nanjing 210014, China
关键词:
圆锥绣球种子形态种子休眠萌发特性发芽率种子污染率
Keywords:
Hydrangea paniculataSeed morphologySeed dormancyGermination characteristic Germinnation rate Contamination rate
分类号:
S604+.1;S685.99
DOI:
10.3969/j.issn.1001-7380.2022.03.002
文献标志码:
A
摘要:
针对圆锥绣球‘石灰灯’(Hydrangea paniculata ‘Limelight’)种子形态特征及萌发特性,测定了种子大小、含水量、吸水率,并研究了贮藏温度与消毒方法对种子萌发的影响。结果表明:‘石灰灯’种子不存在形态及物理休眠。种子成熟时胚发育完全,种皮透水性良好。初始含水量为4.3%,96 h时吸水达饱和状态,吸水率为37.7%;随着贮藏温度下降,种子发芽率呈上升趋势,超低温(-70 ℃)处理下发芽率最高(12.7%),且污染率最低(41.3%);不同消毒方式下, 75% C2H5OH处理组中,种子污染率随消毒时间延长而显著降低,以消毒90 s效果最好,种子污染率和发芽率分别为16.0%和17.3%;15% NaClO处理组中,虽然种子污染率与发芽率随着消毒时间的延长差异不显著,但处理3 min的效果最佳,种子发芽率和污染率分别为22.7%和14.3%。
Abstract:
In this paper, seed morphological and germination characteristics of Hydrangea paniculata ‘Limelight’ were studied, including seed size, water content, water absorption, and the effects of storage temperature and disinfection method on seed germination. The results showed that the seed did not exist morphological or physical dormancy. The embryos was fully develop in the mature seeds and the seed testa had good water permeability. The initial moisture content was 4.3%. After 96 h, water absorption reached saturation and the water absorption rate was 37.7%. With the decrease of storage temperature, the germination rate of seed showed an upward trend. Under the ultra-low temperature treatment (-70 ℃), the germination rate was the highest (12.7%) and the pollution rate was the lowest (41.3%). Under different disinfection treatment methods, the 75% C2H5OH treatment group’s seed contamination rate decreased significantly with the prolongation of the disinfection time, with the best effect, 16.0% seed contamination rate and 17.3% germination rate in 90 s duration disinfection. In the 15% NaClO treatment group, the seed contamination rate and germination rate had not significantly differerence with the prolongation of disinfection time, but the best effect was obtained after disinfection for 3 minutes, and the seed germination rate and contamination rate were 22.7% and 14.3%, respectively.

参考文献/References:

[1]ZHANG S, MA J, SHENG L, et al. Total coumarins from Hydrangea paniculata show renal protective effects in lipopolysaccharide-induced acute kidney injury via anti-inflammatory and antioxidant activities[J]. Frontiers in Pharmacology, 2017, 8: 872.
[2]ZHANG S, YANG J, LI H, et al. Skimmin, a coumarin, suppresses the streptozotocin-induced diabetic nephropathy in wistar rats[J]. European Journal of Pharmacology, 2012, 692(1): 78-83.
[3]赖小连, 颜立红, 颜玉娟, 等.夏蜡梅种子形态结构和种子萌发研究[J].种子, 2021, 40(2): 86-90.
[4]聂秀美. 贮藏年限对燕麦种子种带真菌和真菌毒素的影响[D].兰州:甘肃农业大学, 2020: 1-9.
[5]HARRINGTON J F. Drying, storage, and packaging seed to maintain germination and vigor[J]. Seed Technology Papers,1959: 89-107.
[6]刘燕,周慧,方标.园林花卉种子超低温保存研究[J].北京林业大学学报, 2001, 23(4): 39-44.
[7]乔谦,王江勇,陶吉寒.绣球属植物研究进展[J].农学学报, 2020, 10(4): 60-64.
[8]孙晓波,苏家乐,陈双双,等.大花绣球‘无尽夏’组培苗叶片再生植株的研究[J].中国农学通报, 2020, 36(16): 67-72.
[9]陈双双,齐香玉,冯景,等.基于流式细胞术和基因组Survey的绣球基因组大小及特征分析[J].江苏农业科学,2021,49(12):39-44.
[10]邓衍明,韩勇,齐香玉,等.绣球属植物种质资源分析及其花色可调性和叶斑病抗性比较[J].植物资源与环境学报, 2018, 27(4): 90-100.
[11]陈双双,齐香玉,冯景,等.铝处理下绣球实时荧光定量PCR内参基因筛选及验证[J].华北农学报, 2021, 36(2): 9-18.
[12]ISTA. International rules for seed testing[Z]. Switzerland; The International Seed Testing Association(ISTA). 2005.
[13]ELLIS R H, ROBERTS E H. The quantification of ageing and survival in orthodox seeds[J]. Seed Science and Technology, 1981, 9(2): 373-409.
[14]MAGUIRE I D. Speed of germination-aid in selection and evaluation for seedling emergence and vigor [J]. Crop Science, 1962, 2(2): 176-177.
[15]张梅,刘旭,周惠龙,等.绣球属29种植物的种子微形态研究[J].园艺学报,2018,45(6): 1147-1159.
[16]NIKOLAEVA M G. On criteria to use in studies of seed evolution[J].Seed Science Research, 2007, 14(4): 315-320.
[17]WILLIS C G, BASKIN C C, BASKIN J M, et al. The evolution of seed dormancy: environmental cues, evolutionary hubs, and diversification of the seed plants[J].New Phytologist, 2014, 203(1): 300-309.
[18]吴玉,周亚晶,胡惠,等.种子休眠与解除的分子机制研究进展[J].种子, 2021, 40(5): 63-70.
[19]BASKIN J M, BASKINC C. A classification system for seed dormancy[J].Seed Science Research, 2004, 14(1): 1-16.
[20]BASKIN C C, BASKIN J M. Seeds: ecology, biogeography, and evolution of dormancy and germination[J]. Crop Science, 2014, 40(2): 564.
[21]FIGUEROA J, ARMESTO J J. Community-wide germination strategies in a temperate rainforest of southern chile: ecological and evolutionary correlates[J].Australian Journal of Botany, 2001, 49: 411-425.
[22]GREER S P, RINEHART T A. In vitro gemination and dormancy responses of Hydrangea macrophylla and Hydrangea paniculata seeds to ethyl methane sulfonate and cold treatment[J]. HortScience, 2009, 44(3): 764-769.
[23]LU Y, ZHANG D, YI Y, et al. In vitro seed germination of Hydrangea paniculata Sieb[C]. New Orleans: ASHS, 2015.
[24]王晓静,余乐,殷寿华.授粉方式对流苏石斛结实率及种子活力的影响[J].云南大学学报(自然科学版), 2009, 31(S1): 374-377.
[25]BORBA E L, SEMIR J, SHEPHERD G J. Self-incompatibility, inbreeding depression and crossing potential in five brazilian Pleurothallis (Orchidaceae) species[J]. Annals of Botany, 2001, 88(1): 89-99.
[26]樊莉丽. 楸树生殖生物学特性的研究[D].南京:南京林业大学, 2012:70-78.
[27]ALEXANDER L. Ploidy level influences pollen tube growth and seed viability in interploidy crosses of Hydrangea macrophylla[J]. Frontiers in Plant Science, 2020, 11:100.
[28]REED S M. Compatibility studies in Hydrangea[J]. Journal of Environmental Horticulture, 2000, 18(1): 29-33.
[29]陆乙卜,龚记熠,肖松,等.圆锥绣球种子离体萌发的培养条件[J].贵州农业科学, 2015, 43(11): 34-37.
[30]董文科,马晖玲,陈春艳.不同消毒剂对甘肃红豆草种子消毒效果及萌发的影响[J].草原与草坪, 2015, 35(4): 80-83.
[31]REED S M. Development of an in ovolo embryo culture procedure for Hydrangea[J]. Journal of Environmental Horticulture, 2000, 18(1): 34-39.

备注/Memo

备注/Memo:
收稿日期:2022-03-20;修回日期:2022-04-06
基金项目:江苏省农业科技自主创新资金项目“绣球与乔木绣球种间杂交胚拯救技术研究”(CX(21)3022);江苏省林业科技创新与推广项目“绣球耐寒新品种引选与花色定制产品培育利用研究”(LYKJ-沭阳[2021]01);苏州市科技计划项目“绣球花色定制产品高效生产关键技术应用研究”(SNG201923);江苏省种业振兴“揭榜挂帅”项目“绣球花优质多抗新品种选育”(JBGS[2021]097)
作者简介:冯景(1989- ),男,江苏睢宁人,助理研究员,博士。研究方向为花卉种质创新与栽培生理。E-mail:fengj@jaas.ac.cn
*通信作者:邓衍明(1976- ),男,安徽滁州人,研究员,博士。研究方向为观赏植物遗传育种与栽培。E-mail:nksdym@163.com
更新日期/Last Update: 2022-08-08