[1]方景涛,敬辽,罗志力,等.外源脱落酸对干旱胁迫下杨树生长和生理特性的影响[J].江苏林业科技,2020,47(05):11-17.[doi:10.3969/j.issn.1001-7380.2020.05.003]
　Fang Jingtao,Jing Liao,Luo Zhili,et al.Effect of exogenous abscisic acid on the growth and physiological properties of poplar under drought stress[J].Journal of Jiangsu Forestry Science &Technology,2020,47(05):11-17.[doi:10.3969/j.issn.1001-7380.2020.05.003]
点击复制

外源脱落酸对干旱胁迫下杨树生长和生理特性的影响()
分享到：

《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第47卷
期数:
2020年05期
页码:
11-17
栏目:
试验研究
出版日期:
2020-10-31

文章信息/Info

Title:
Effect of exogenous abscisic acid on the growth and physiological properties of poplar under drought stress
文章编号:
1001-7380（2020）05-0011-07
作者:
方景涛1; 敬辽2; 罗志力1; 毕凌聆1; 毕凌凤1; 冯宇虹1; 陈刚1*
1.四川农业大学 林学院，四川成都611130；
2.四川省林业和草原调查规划院，四川成都610081
Author(s):
Fang Jingtao1;  Jing Liao2;  Luo Zhili1;  Bi Lingling1;  Bi Lingfeng1;  Feng Yuhong1;  Chen Gang1*
1. College of Forestry, Sichuan Agricultural University，Chengdu 611130，China；
2. Sichuan Forestry and Grassland Survey and Planning Institute, Chengdu 610081, China
关键词:
生理响应; 杨树; 脱落酸; 干旱胁迫
Keywords:
Physiological response; Poplar; Abscisic acid; Drought stress
分类号:
Q945.78；Q946.885+.6；S792.119
DOI:
10.3969/j.issn.1001-7380.2020.05.003
文献标志码:
A
摘要:
为研究不同质量浓度外源脱落酸（ABA）对处于不同干旱时期杨树幼苗抗性生理的影响，选用当年生北京杨扦插幼苗每隔3 d共计喷施4次0（对照）、10（低）、50（中）和100（高）mg/L ABA后，测定自然干旱第0，5，10天时杨树地径增长量、株高增长量、生物量、过氧化氢酶、过氧化物酶和超氧化物岐化酶活性，及过氧化氢、丙二醛、可溶性蛋白和可溶性糖含量。结果表明：土壤含水量随着干旱时间的增长而不断降低，叶片含水量也呈显著下降趋势，但在干旱10 d时，高质量浓度处理的杨树叶片含水量高于对照。杨树植株的地径增长量和地下生物量虽然未受到ABA的显著影响，但低中质量浓度处理下的株高增长量显著增加。酶促活性氧清除系统中，中高质量浓度的ABA在干旱胁迫严重的第10天增加了超氧化物岐化酶的活性，但过氧化氢和丙二醛没有受到ABA的影响。在杨树受到严重干旱胁迫的第10天，低质量浓度ABA显著增加了渗透调节物质可溶性蛋白的浓度。该研究表明在较严重的干旱强度下，低中质量浓度ABA主要通过提高活性氧清除酶中超氧化物岐化酶活性和渗透物质可溶性蛋白含量来提高杨树的抗旱能力。
Abstract:
In order to study the physiological responses in poplar under drought stress after exogenous abscisic acid (ABA) treatment, cutting seedlings of the current year poplar were sprayed with 4 times every 3 days of 0 (CK), 10 (low), 50 (medium) and 100 (high) mg/L ABA. The basal diameter increment, plant height increment, biomass, catalase, peroxidase, superoxide dismutase, hydrogen peroxide, malondialdehyde, soluble protein and soluble sugar content were determined on day 0, 5, and 10 following a natural drought. The results showed that the soil water content decreased with the increase of the drought duration, and the leaf relative water content also showed a significant downward trend. However, the leaf relative water content of the poplar treated with high concentration was higher than that of the control ones on day 10. The basal diameter increment and underground biomass of poplar seedlings were not significantly affected by ABA, but the height increment was significantly increased. In the enzymatic active oxygen scavenging system, ABA treatment at medium and high concentrations increased the activity of superoxide dismutase on the day 10 under severe drought stress. But H2O2 or malondialdehyde were not affected by ABA. On day 10, when poplar was severely stressed by drought, ABA at low concentration significantly increased the concentration of soluble protein in osmotic adjustment. This study indicated that under severe drought intensity, the increased drought resistance by ABA at low and medium concentration is mainly resulted from the increase of the activity of superoxide dismutase in enzymatic active oxygen scavenging system and the concentration of the adjustment of osmotic substance soluble protein in poplar.

参考文献/References:

[1]MAESTRE F T,VALLADARES F,REYNOLDS J F.Is the change of plant-plant interactions with abiotic stress predictable? A meta-analysis of field results in arid environments [J]. Journal of Ecology, 2005, 93(4):748-757.
[2]李吉跃.植物耐旱性及其机理[J].北京林业大学学报,1991,13(3):92-100.
[3]包秀霞,包秀平,廉勇.干旱胁迫对内蒙古草原多根葱生理生化指标的影响[J].华北农学报,2017,32(1):233-238.
[4]FUJITA Y,FUJITA M,SATOH R, et al. AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis[J].The Plant Cell,2005,17(12):3470-3488.
[5]郝格格,孙忠富,张录强,等.脱落酸在植物逆境胁迫研究中的进展[J].中国农学通报,2009,25(18):212-215.
[6]李跃,万里强,李向林.内源脱落酸生理作用机制及其与苜蓿耐旱性关系研究进展[J].草业学报,2015,24(11):195-205.
[7]闫志利,牛俊义,席玲玲,等.水分条件对豌豆保护酶活性及膜脂过氧化的影响[J].中国生态农业学报,2009,17(3):554-559.
[8]王岩磊.复水与外源脱落酸处理对干旱胁迫下猕猴桃幼苗抗旱性的影响[D].咸阳:西北农林科技大学, 2010.
[9]阮英慧,董守坤,刘丽君,等.外源脱落酸对大豆花期抗旱生理特性的影响[J].耕作与栽培,2014,1(1):6-8.
[10]魏鑫,倪虹,张会慧,等.外源脱落酸和油菜素内酯对干旱胁迫下大豆幼苗抗旱性的影响[J].中国油料作物学报,2016,38(5):605-610.
[11]TWORKOSKI T,WISNIEWSKI M,ARTLIP T.Application of BABA and s-ABA for drought resistance in apple[J].Journal of Applied Horticulture,2011,13(2):85-90.
[12]YIN C Y,DUAN B L,WANG X,et al.Morphological and physiological responses of two contrasting Poplar species to drought stress and exogenous abscisic acid application[J].Plant Science,2004,167(5):1091-1097.
[13]HU Y,WANG B,HU T X,et al.Combined action of an antioxidant defence system and osmolytes on drought tolerance and post-drought recovery of Phoebe zhennan S. Lee saplings[J].Acta Physiologiae Plantarum,2015,37(4):84.
[14]熊庆娥.植物生理学实验教程[M].成都:四川科学技术出版社,2003.
[15]REDDY A R,CHAITANYA K V,VIVEKANANDAN K.Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants[J].Journal of Plant Physiology,2004,161(11):1189-1202.
[16]WU H L,WU X L,LI Z H,et al.Physiological evaluation of drought stress tolerance and recovery in Cauliflower (Brassica oleracea L.) seedlings treated with methyl jasmonate and coronatine[J].Journal of Plant Growth Regulation,2012,31(1):113-123.
[17]NOCTOR G,FOYER C H.ASCORBATE AND GLUTATHIONE:keeping active oxygen under control[J].Annual Review of Plant Physiology and Plant Molecular Biology,1998,49(1):249-279.
[18]ULLAH A,SUN H,YAN X,et al.A novel cotton WRKY gene,GhWRKY6-like, improves salt tolerance by activating the ABA signaling pathway and scavenging of reactive oxygen species[J].Physiologia Plantarum,2018,162(4):439-454.
[19]LAMBERS H,SHANE M W,CRAMER M D,et al.Root structure and functioning for efficient acquisition of phosphorus: matching morphological and physiological traits[J].Annals of Botany,2006,98(4):693-713.
[20]康俊梅,杨青川,樊奋成.干旱对苜蓿叶片可溶性蛋白的影响[J].草地学报,2005,13(3):199-202.
[21]SILVA E N,FERREIRA-SILVA S L,VIEGAS R A,et al.The role of organic and inorganic solutes in the osmotic adjustment of drought-stressed Jatropha curcas plants[J].Environmental and Experimental Botany,2010,69(3):279-285.
[22]李丽杰,顾万荣,张倩,等.外源亚精胺对干旱胁迫下玉米幼苗叶片生理及根系特征参数的影响[J].农药学学报,2015,17(3):291-299.
[23]BRITO C,DINIS L T,FERREIRA H, et al. Salicylic acid increases drought adaptability of young olive trees by changes on redox status and ionome[J].Plant Physiology and Biochemistry,2019,141: 315-324.
[24]MARCINSKA I,CZYCZYLO-MYSZA I,SKRZYPEK E,et al.Alleviation of osmotic stress effects by exogenous application of salicylic or abscisic acid on wheat seedlings[J].International Journal of Molecular Sciences,2013,14(7):13171-13193.

相似文献/References:

[1]夏采意,薛毅民,苏 杰,等.圩堤杨树防护林桑天牛发生及综合防治效果[J].江苏林业科技,2015,42(02):47.[doi:10.3969/j.issn.1001-7380.2015.02.011]
[2]纪纯阳.杨树基因工程育种研究进展[J].江苏林业科技,2015,42(02):50.[doi:10.3969/j.issn.1001-7380.2015.02.012]
　JI Chun-yang.Research advances on genetic engineering breeding of poplar[J].Journal of Jiangsu Forestry Science &Technology,2015,42(05):50.[doi:10.3969/j.issn.1001-7380.2015.02.012]
[3]江浩,季永华*,王磊,等.杨农复合经营系统碳储量及其分布特征研究[J].江苏林业科技,2015,42(03):28.[doi:10.3969/j.issn.1001-7380.2015.03.007]
　JIANG Hao,JI Yong-hua*,WANG Lei,et al.Carbon storage and distribution in poplar and poplar-crop intercropping forests[J].Journal of Jiangsu Forestry Science &Technology,2015,42(05):28.[doi:10.3969/j.issn.1001-7380.2015.03.007]
[4]江浩,何冬梅,季永华,等.杨农复合经营系统不同土壤层养分分布研究[J].江苏林业科技,2016,43(01):10.[doi:10.3969/j.issn.1001-7380.2016.01.003]
　JIANG Hao,HE Dong-mei,JI Yong-hua,et al.Depth distribution difference of soil nutrient elements between poplar forest and poplar-crop intercropping pattern[J].Journal of Jiangsu Forestry Science &Technology,2016,43(05):10.[doi:10.3969/j.issn.1001-7380.2016.01.003]
[5]唐凌凌,徐长柏,曹国华,等.8个杨树无性系1年生扦插苗年生长节律研究[J].江苏林业科技,2016,43(01):15.[doi:10.3969/j.issn.1001-7380.2016.01.004]
[6]涂慧珍,王 硕*,汤 方**.辛硫磷和氟虫腈对杨树多酚氧化酶活性的影响[J].江苏林业科技,2016,43(03):9.[doi:10.3969/j.issn.1001-7380.2016.03.003]
　TU Hui-zhen,WANG Shuo*,TANG Fang**.Effect of phoxim or fipronil spray on polyphenol oxidase activity in poplar leaves[J].Journal of Jiangsu Forestry Science &Technology,2016,43(05):9.[doi:10.3969/j.issn.1001-7380.2016.03.003]
[7]刘云鹏,巩 睿,解春霞,等.基于Landsat遥感影像的杨树信息提取方法分析[J].江苏林业科技,2017,44(02):28.[doi:10.3969/j.issn.1001-7380.2017.02.007]
　LIU Yun-peng,GONG Rui,XIE Chun-xia,et al.Study on information extraction of poplar resources using Landsat remotely sensed datasets[J].Journal of Jiangsu Forestry Science &Technology,2017,44(05):28.[doi:10.3969/j.issn.1001-7380.2017.02.007]
[8]高 悦,李 刚,杨运具,等.飞机喷洒甲维·灭幼脲的雾滴参数测定及其防治美国白蛾效果的研究[J].江苏林业科技,2017,44(03):45.[doi:10.3969/j.issn.1001-7380.2017.03.011]
　GAO Yue,LI Gang,YANG Yun-ju,et al.Application research of Hyphantria cunea Drury prevention and control using emamectin benzoate-chlorbenzuron by airplane[J].Journal of Jiangsu Forestry Science &Technology,2017,44(05):45.[doi:10.3969/j.issn.1001-7380.2017.03.011]
[9]丁晶晶,樊向民,胡高瞻,等.泗阳县杨树林下种植雪菜经济效益分析[J].江苏林业科技,2017,44(06):21.[doi:10.3969/j.issn.1001-7380.2017.06.006]
[10]施士争,路 明,王红玲,等.江苏苏北杨树农田林网更新主栽树种选择研究[J].江苏林业科技,2017,44(06):27.[doi:10.3969/j.issn.1001-7380.2017.06.008]

备注/Memo

备注/Memo:
收稿日期：2020-08-08;修回日期：2020-08-20
基金项目：四川农业大学科研启动费（1922996004）；四川农业大学大学生科研兴趣培养计划“外源脱落酸对杨树干旱抗性生理生化的影响”（2021996452）
作者简介：方景涛（1998- ），男，四川成都人，大学本科生。E-mail：1229551668@qq.com。
*通信作者：陈刚（1989- ），男，四川巴中人，副教授，博士。主要从事林木栽培生理方面的研究工作。E-mail：g.chen@sicau.edu.cn。
更新日期/Last Update: 2020-12-09