[1]李千惠,张丹丹,姜文龙,等.‘高原红’海棠花粉特性与生活力测定研究[J].江苏林业科技,2018,45(02):1-5.[doi:10.3969/j.issn.1001-7380.2018.02.001]
　LI Qian-hui,ZHANG Dan-dan,JIANG Wen-long,et al.Study on pollen characteristics and vigor of ornamental crabapple[J].Journal of Jiangsu Forestry Science &Technology,2018,45(02):1-5.[doi:10.3969/j.issn.1001-7380.2018.02.001]
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‘高原红’海棠花粉特性与生活力测定研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第45卷
期数:
2018年02期
页码:
1-5
栏目:
试验研究
出版日期:
2018-04-30

文章信息/Info

Title:
Study on pollen characteristics and vigor of ornamental crabapple
文章编号:
1001-7380（2018）02-0001-05
作者:
李千惠1; 张丹丹1; 姜文龙1; 薛源1; 张往祥1; 2; 王改萍1*
1. 南方协同中心,南京林业大学林学院，江苏南京210037；
2. 扬州小苹果园艺有限公司，江苏扬州225200
Author(s):
LI Qian-hui1;  ZHANG Dan-dan1;  JIANG Wen-long1;  XUE Yuan1;  ZHANG Wang-xiang1; 2;  WANG Gai-ping1*
1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. Yangzhou Crabapple Horticulture Limited Company, Yangzhou 225200, China
关键词:
观赏海棠; 花粉离体培养; 花粉活体萌发; 花粉贮藏
Keywords:
Ornamental crabapple;  Pollen in vitro culture;  Pollen in vitro germination;  Pollen storage
分类号:
Q944.42；S685.99
DOI:
10.3969/j.issn.1001-7380.2018.02.001
文献标志码:
A
摘要:
以5年生观赏海棠‘高原红’嫁接苗为试验材料，采用扫描电镜、花粉离体培养、荧光显微技术等研究了‘高原红’花粉的形态、生活力及贮藏特性，分析了花粉离体培养基成分、离体与活体萌发的区别及花粉贮藏过程中活力的变化。探讨了花粉萌发及贮藏特性，旨在为揭示花粉萌发特性、建立花粉贮藏体系提供理论依据。结果表明：硼酸与蔗糖溶液均对‘高原红’花粉萌发有显著的影响，单因素最佳培养基为10%蔗糖溶液；离体与活体花粉在萌发时间上有一定程度的差异，离体萌发较快于活体萌发，且活体萌发中花粉管在授粉后第8—12 h为快速伸长期；花粉贮藏过程中，室温（17—20 ℃）下贮藏的花粉活力急速下降，花粉管长度与4，-20 ℃及-70 ℃时差异显著，4 ℃下贮藏的花粉离体萌发率同样呈急速下降的趋势，与-20，-70 ℃时差异显著，而-20 ℃与-70 ℃下贮藏的花粉其离体萌发率之间与花粉管长度之间差异均不显著。
Abstract:
In this study, with 5-year-old ornamental crabapple Malus ‘prairifire’ grafted seedlings taken as the material, the morphology, viability and storage characteristics of Malus ‘prairifire’ pollen were studied by scanning electron microscopy, in vitro culturing pollen and fluorescence microscopy. The differences of pollen in vitro culture medium, in vitro and in vivo germination and the changes of vitality during pollen storage were analyzed. The pollen germination and storage characteristics were discussed, aiming at providing the theoretical basis for revealing pollen germination characteristics and establishing pollen storage system. The results showed that both boric acid and sucrose solution had significant effects on pollen germination of M. ‘prairifire’. The single factor optimal medium was 10% sucrose solution. The germination time of in vitro pollen varied with that of in vivo pollen to a certain degree, e.g., in vitro germination faster than in vivo germination, and during 8—12 h after pollination, the pollen tube of in vivo germination was in rapid extension; In the pollen storage process, the pollen viability stored at room temperature (17—20 ℃) decreased rapidly, the length of pollen tube was significantly different from those of 4, -20 ℃ and -70 ℃ (P <0.01) and the germination rate of pollen in vitro was also decreased rapidly at 4 ℃, which was significantly different from that of -20 ℃ or -70 ℃ (P <0.01), while there was no significant difference in the germination rate and pollen tube length between the pollen stored at -20 ℃ and -70 ℃ (P＜0.01).

参考文献/References:

［1］MICHAEL A. Dirrmanual of woody landscape plants: their Indentification, ornamental characteristics, culture, propagation and uses[M]. Ed. 4. Champaign Illinois: Stipes Publishing Company, 1990: 12-15.
［2］ROBINSON J P, HARRIS S A, JUNIPER B E. Taxonomy of the genus Malus Mill.(Rosaceae)with emphasis on the cultivated apple, M. domestica Borkh.[J]. Plant Systematics & Evolution, 2001, 226(1-2): 35-58.
［3］PARTON E, VERVAEKE I, DELEN R, et al. Viability and storage of bromeliad pollen[J]. Euphytica, 2002, 125(2):155-161.
［4］DUTTA S K, SRIVASTAV M, CHAUDHARY R, et al. Low temperature storage of mango (Mangifera indica, L.) pollen[J]. Scientia Horticulturae, 2013, 161(2):193-197.
［5］NOVARA C, ASCARI L, MORGIA V L, et al. Viability and germinability in long term storage of Corylus avellana pollen[J]. Scientia Horticulturae, 2017, 214:295-303.
［6］BHAT Z A, DHILLON W S, SHAFI R H S, et al. Influence of storage temperature on viability and in vitro germination capacity of pear (Pyrus spp.) pollen[J]. Journal of Agricultural Science, 2012, 4(11):128-135.
［7］DAFNI A, FIRMAGE D. Pollen viability and longevity: Practical, ecological and evolutionary implications[J]. Plant Systematics & Evolution, 2000, 222(1):113-132.
［8］WANG Q L, LU L D, WU X Q, et al. Pollen preservation and its viability test[J]. Chinese Bulletin of Botany, 2002, 19(3):365-373.
［9］PERVEEN A, KHAN S A. Maintenance of pollen germination capacity of Malus pumila L. (Rosaceae)[J]. Pakistan Journal of Botany, 2008, 40(3):963-966.
［10］SPARKS D. Abnormal flowering in pecan associated with freezing temperature[J]. Hortscience A Publication of the American Society for Horticultural Science, 1992, 27(7):801-803.
［11］STANLEY R G, LINSKENS H F. Pollen: biology biochemistry management[M]. Springer: Science & Business Media, 2012.
［12］程伟, 赵志刚, 曾杰,等. 西南桦花粉离体萌发的适宜条件与萌发特征[J]. 林业科学研究, 2007, 20(2):209-212.
［13］李志能, 刘国锋, 罗春丽,等. 悬铃木花粉生活力及贮藏力的研究[J]. 植物科学学报, 2006, 24(1):54-57.
［14］龚双姣, 马陶武, 刘强. 培养基组分及培养条件对蜡梅花粉萌发及花粉管生长的影响[J]. 西北植物学报, 2012, 32(6):1254-1260.
［15］刘雪莲, 李庆玲, 秦佳梅,等. 不同培养基对紫丁香花粉离体萌发的影响[J]. 东北林业大学学报, 2010, 38(7):61-63.
［16］杜纪红, 叶正文, 苏明申,等. 桃花粉离体萌发和花粉管生长特性研究[J]. 西北植物学报, 2011, 31(1):64-71.
［17］金亚征, 忻龙祚, 王建民,等. 培养基成分对仁用杏花粉离体萌发的影响[J]. 经济林研究, 2014, 32(1):101-106.
［18］何春燕, 谭晓风, 袁德义,等. 7种油茶花粉数量及花粉萌发率的测定[J]. 中南林业科技大学学报, 2009, 29(1):74-78.
［19］刘鹏, 张太奎, 王连春,等. 牛角瓜花粉离体萌发研究[J]. 经济林研究, 2015, 33(2):142-148.
［20］胡适宜. 被子植物胚胎学[M]. 北京:高等教育出版社(人民教育出版社), 1982.
［21］周蕴薇, 刘芳, 李俊涛. 百合花粉生活力及贮藏特性[J]. 东北林业大学学报, 2007, 35(5):39-40.
［22］高芬, 沈永宝. 南京椴花粉贮藏寿命的研究[J]. 南京林业大学学报(自然科学版), 2009, 33(2):141-144.
［23］查钱慧, 谭莎, 黄永芳,等. 5个油茶无性系花粉贮藏特性及杂交亲和力的研究[J]. 中南林业科技大学学报, 2015, 35(10):79-82.

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备注/Memo

备注/Memo:
收稿日期：2018-02-05;修回日期：2018-03-01
基金项目：江苏省重点研发（现代农业）项目“南方型观赏海棠特异种质挖掘与良种选育”(BE2016388)；江苏省农业科技自主创新项目“优新树种选育与高效栽培技术方案”[cx(16)1005]
作者简介：李千惠（1992- ），女，江苏徐州人，硕士研究生。
*通信作者：王改萍（1970- ），女，山西榆社人，副教授，博士。主要从事林木生理生化方面的研究。E-mail: wanggaiping@njfu.edu.cn。
更新日期/Last Update: 2018-05-23