[1]于艳华,郭加忠,郭同斌*,等.白蛾黑基啮小蜂寄生对杨小舟蛾蛹血细胞包囊反应的影响及其毒液对蛹的毒性研究[J].江苏林业科技,2015,42(03):1-5.[doi:10.3969/j.issn.1001-7380.2015.03.001]
 YU Yan-hua,GUO Jia-zhong,GUO Tong-bin*,et al.Effect of parasitism of Tetrastichus nigricoxae Yang on the encapsulationresponse of its host,Micromelalopha troglodyta Graeser pupaeand toxicity of its venom to pupal host[J].Journal of Jiangsu Forestry Science &Technology,2015,42(03):1-5.[doi:10.3969/j.issn.1001-7380.2015.03.001]
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白蛾黑基啮小蜂寄生对杨小舟蛾蛹血细胞包囊反应的影响及其毒液对蛹的毒性研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第42卷
期数:
2015年03期
页码:
1-5
栏目:
试验研究
出版日期:
2015-06-30

文章信息/Info

Title:
Effect of parasitism of Tetrastichus nigricoxae Yang on the encapsulationresponse of its host,Micromelalopha troglodyta Graeser pupaeand toxicity of its venom to pupal host
文章编号:
1001-7380(2015)03-0001-05
作者:
于艳华1郭加忠2郭同斌1*宋明辉1颜学武3
1.徐州市森林病虫防治检疫站,江苏 徐州 221009;
2. 高邮市京杭运河管理处,江苏 高邮 225600;
3.湖南省林业科学研究院,湖南 长沙 410004;
Author(s):
YU Yan-hua1GUO Jia-zhong2 GUO Tong-bin11* SONG Ming-hui1YAN Xue-wu3
1. Forest Pest Management and Quarantine Station of Xuzhou,Xuzhou 221009,China;
2. Gaoyou Administration Office of Grand Canal,Gaoyou 225600,China;
3. Hunan Academy of Forestry,Changsha 410004,China
关键词:
白蛾黑基啮小蜂 杨小舟蛾 包囊反应 毒液
Keywords:
Tetrastichus nigricoxae Yang Micromelalopha troglodyta Graeser Encapsulation response Venom
分类号:
Q968
DOI:
10.3969/j.issn.1001-7380.2015.03.001
文献标志码:
A
摘要:
测定了白蛾黑基啮小蜂(Tetrastichus nigricoxae)的寄生对寄主杨小舟蛾(Micromelalopha troglodyta)蛹血细胞包囊反应的影响及其毒液对蛹的毒性。结果表明,杨小舟蛾蛹血细胞具有很强的包囊异物能力,而在白蛾黑基啮小蜂毒液的作用下这种能力会丧失,因而白蛾黑基啮小蜂的卵能在寄主蛹中顺利完成世代发育,而不被包囊。Sephadex A-25珠被注入蛹内24 h后的被包囊率高达94.00%。白蛾黑基啮小蜂寄生早期(寄生0~2 h后)不影响寄主蛹的血细胞免疫反应,寄生后期(寄生24,48 h后)杨小舟蛾蛹的血细胞免疫反应受到较大影响。γ射线辐射处理的白蛾黑基啮小蜂寄生蛹包囊能力的变化与正常白蛾黑基啮小蜂寄生蛹相似,随着寄生后时间延长而下降。白蛾黑基啮小蜂毒液对杨小舟蛾蛹的血细胞免疫反应有抑制作用,且具有一定的毒性。
Abstract:
Experiments were made to determine the effect of parasitism of Tetrastichus nigricoxae Yang on the encapsulationresponse of its host,Micromelalopha troglodyta Graeser pupae and toxicity of its venom to pupal host. We found out that thehemocyte-mediated encapsulation towards the injected Sephadex A-25 beads in the pupae of M.troglodyta was as high as94. 00%. This ability would lose after being parasitised by T. nigricoxae Yang. Parasitism did not affect in vivo encapsulationresponse of pupae to injected Sephadex A-25 beads in early stage ( 0 ~ 2 h after parasitism) ,but did affect the responsein later stage ( 24 and 48 h after parasitism) . Pseudoparasitism trigged by C60-γ ray irradiation influenced host’s encapsulationin a similar way as normal parasitism,and the parsitized and pseudoparasitized pupae both died about 48 h afterparasitism. The injection of venom could lead the recipient pupae to death,confirming a toxic action of venom.

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备注/Memo

备注/Memo:
收稿日期:2015-04-20; 修回日期:2015-04-28
基金项目:江苏省“333工程”科研项目(BRA2010027)
作者简介:于艳华(1967- ),女,江苏新沂人,高级工程师,从事林业有害生物防治技术推广工作。
*通信作者:郭同斌(1967- ),男,研究员级高级工程师,博士。E-mail:gtb1101@163.com。
更新日期/Last Update: 2015-08-20