[1]徐丽丽,解春霞,郑华英,等.松墨天牛被管氏肿腿蜂寄生后体内蛋白质含量的变化[J].江苏林业科技,2018,45(06):22-25.[doi:10.3969/j.issn.1001-7380.2018.06.006]
　Xu Lili,Xie Chunxia,Zheng Huaying,et al.Changes in protein contents of Monochamus alternatus (Coleoptera: Cerambycidae) larvae parasitized by Sclerodermus guani (Hymenoptera: Chrysidoidea)[J].Journal of Jiangsu Forestry Science &Technology,2018,45(06):22-25.[doi:10.3969/j.issn.1001-7380.2018.06.006]
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松墨天牛被管氏肿腿蜂寄生后体内蛋白质含量的变化()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第45卷
期数:
2018年06期
页码:
22-25
栏目:
试验研究
出版日期:
2018-12-30

文章信息/Info

Title:
Changes in protein contents of Monochamus alternatus (Coleoptera:
Cerambycidae) larvae parasitized by Sclerodermus guani (Hymenoptera: Chrysidoidea)
文章编号:
1001-7380（2018）06-0022-04
作者:
徐丽丽; 解春霞; 郑华英; 刘云鹏
江苏省林业科学研究院，江苏 南京 211153
Author(s):
Xu Lili;  Xie Chunxia;  Zheng Huaying;  Liu Yunpeng
Jiangsu Academy of Forestry，Nanjing 211153, China
关键词:
管氏肿腿蜂; 寄主调控; 外寄生蜂; 松墨天牛; 蛋白质代谢
Keywords:
Sclerodermus guani ;  Host regulation;  Ectophytic parasitoid;  Monochamus alternatus ;  Protein metabolism
分类号:
O629.73；S763.38;S763.43
DOI:
10.3969/j.issn.1001-7380.2018.06.006
文献标志码:
A
摘要:
管氏肿腿蜂（Sclerodermus guani）是多种林木钻蛀性害虫的体外寄生蜂，雌蜂通过蜇刺注射毒液和卵巢蛋白对寄主体内的营养物质进行调节。为明确肿腿蜂寄生后松墨天牛体内各部位蛋白质含量的变化，使用考马斯亮蓝法对初始组，第2天（寄主初麻痹时）、第14天（幼蜂初孵时）的处理组和对照组的松墨天牛虫体、表皮、血淋巴和脂肪体的蛋白质含量进行测定。结果表明：处理组松墨天牛虫体和脂肪体内的蛋白质含量在第2天均显著高于初始组，虫体蛋白质含量在第14天显著低于第2天，与同期对照组相反。处理组松墨天牛的虫体、脂肪体和血淋巴中蛋白质含量在第2天皆显著高于初始组，而表皮则相反；虫体与脂肪体蛋白质含量显著低于第2天，而表皮与血淋巴则相反；虫体的变化与脂肪体的变化趋势相同。该研究结果为进一步了解雌蜂对寄主的调控机理提供了一定的理论基础。
Abstract:
Sclerodermus guani is the ectophytic parasitoid of many wood-boring insects. The females inject venom and ovarian proteins by sting to adjust the nutrient metabolism of the hosts. The total protein contents in the larva, cuticle, hemolymph and fat body of the host insects of control group and parasitized group on day 2 and 14, and initial group were examined with Bradford's method, to investigate the changes of the protein contents in different sections of the host larvae before and after parasitism. The results were as follows: The protein contents in the larva and fat body of parasitized alternative host larvae on the second day, were significantly higher than those of the initial group. And lower level of the protein contents in the larva were observed on the 14th day than on the second day. These were contrary to the variations of the control group without parasitism at the same period. The protein contents in the larva, fat body and hemolymph of parasitized alternative host larvae on the second day, were significantly higher than those of the initial group, which was contrary in cuticle. The protein contents in the larva and fat body on the 14th day were significantly lower than on the second day, which was contrary in hemolymph. The variations of the protein contents were similar between the larva and fat body. Our results will provide a basis for further studies on the adjusting and controlling mechanism of the parasitoid to the host.

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备注/Memo

备注/Memo:
收稿日期：2018-10-26;修回日期：2018-11-25
基金项目：中央高校基本业务费专项资金
作者简介：徐丽丽（1991- ），女，浙江台州人，硕士。主要研究方向：森林保护学。E-mail:jiangfeimeng@126.com。
*通信作者：徐丽丽（1991- ），通讯地址：江苏省南京市江宁区丹阳大道109号，江苏省林业科学研究院。E-mail:jiangfeimeng@126.com。电话：13913905746。
更新日期/Last Update: 2019-02-20