[1]王伟伟,姚玲,何开跃,等.不同柳树花粉离体培养及活力测定方法比较研究[J].江苏林业科技,2018,45(04):1-7.[doi:10.3969/j.issn.1001-7380.2018.04.001]
　Wang Weiwei,Yao Ling,He Kaiyue,et al.Comparative study on in vitro pollen germination and viability evaluation of different willow species[J].Journal of Jiangsu Forestry Science &Technology,2018,45(04):1-7.[doi:10.3969/j.issn.1001-7380.2018.04.001]
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不同柳树花粉离体培养及活力测定方法比较研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第45卷
期数:
2018年04期
页码:
1-7
栏目:
试验研究
出版日期:
2018-08-15

文章信息/Info

Title:
Comparative study on in vitro pollen germination and viability evaluation of different willow species
文章编号:
1001-7380（2018）04-0001-07
作者:
王伟伟1; 2; 3; 姚玲3; 何开跃3; 王保松1; 2;  何旭东1; 2*
1.江苏省林业科学研究院，江苏南京210014；
2. 江苏省农业种质资源保护与利用平台， 江苏南京210014；
3. 南京林业大学林学院，江苏南京210037
Author(s):
Wang Weiwei1; 2; 3;  Yao Ling 3;  He Kaiyue3;  Wang Baosong1; 2 ; He Xudong1; 2*
1.Jiangsu Academy of Forestry, Nanjing 211153, China;
2. Willow Nursery of the Jiangsu Provincial Platformfor Conservation and Utilization of Agricultural Germplasm, Nanjing 211153,China;
3.College of Forestry, Nanjing Forestry University, Nanjing 210037, China
关键词:
柳树; 柳属; 离体培养; 花粉活力; 快速检测体系
Keywords:
Willow;  Salix;  In vitro culture;  Pollen viability;  Rapid determination system
分类号:
Q944.42； S792.12
DOI:
10.3969/j.issn.1001-7380.2018.04.001
文献标志码:
A
摘要:
以6个不同种柳树亲本及杂交子代个体为试验材料，采集其花粉进行离体培养以确定最佳培养条件，同时比较离体培养法、蓝墨水染色法、I2-KI染色法和TTC染色法4种花粉活力测定方法，以期探索适合柳树不同种个体材料的花粉活力快速检测体系。结果表明：离体培养基中蔗糖、H3BO3和Ca(NO1)2的质量浓度配比因柳树个体不同而略有差异；不同培养时间对柳树花粉活力影响显著，适宜培养时间为10—12 h; 光照条件对离体培养影响不显著；不同个体最适培养温度均为25 ℃。4种测定方法均可对柳树花粉进行活力检测，蓝墨水染色法可代替离体培养法对部分柳树个体的花粉活力进行快速测定。该研究揭示了柳树不同种个体间花粉变异特点，为柳树杂交育种工作提供了技术支持。
Abstract:
The fresh pollens were collected to optimize the in vitro germination condition from six willow species of different parents and hybrid progeny individuals which were used as experimental materials. And four methods including in vitro culture method, blue ink staining method, I2-KI staining method and TTC staining method, were compared to explore the rapid evaluation system of pollen viability suitable for different willow individuals. The results showed that the concentration of sucrose, H3BO3 and Ca(NO3)2 in media had slightly different effects among individuals. The culture time had significant effects on pollen viability, with the suitable time of 10—12 h. However, the light condition had not significant effect on the culture and the optimum culture temperature was 25 °C. All of the methods for pollen viability evaluation were effective and the blue ink staining method as a fast examination method could take place of in vitro culture method for some willow individuals. This study revealed the characteristics of pollen variation among different willow species, and provided technical support for the willow hybrid breeding.

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备注/Memo

备注/Memo:
收稿日期：2018-07-12;修回日期：2018-08-15
基金项目：江苏省农业科技自主创新资金项目“观赏柳树新品种选育”[cx(14)2024]；江苏省“六大人才高峰”资助项目(2016-NY-036)；江苏省“333高层次人才培养工程”资助项目(BRA2017518)
作者简介：王伟伟(1986 - )，女，河南长垣人，助理研究员，硕士。主要从事植物学研究。
*通信作者：何旭东(1981 - )，男，江苏句容人，副研究员，博士。主要从事林木遗传育种研究。E-mail：hxd_519@163.com。
更新日期/Last Update: 2018-09-13