[1]何丽斯,肖政*,苏家乐,等.羊踯躅花期花瓣类胡萝卜素的含量变化[J].江苏林业科技,2017,44(05):18-21.[doi:10.3969/j.issn.1001-7380.2017.05.004]
　HE Li-si,XIAO Zheng*,SU Jia-le,et al.Changes of carotenoids content in flower petal of Rhododendron molle during flower development[J].Journal of Jiangsu Forestry Science &Technology,2017,44(05):18-21.[doi:10.3969/j.issn.1001-7380.2017.05.004]
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羊踯躅花期花瓣类胡萝卜素的含量变化()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第44卷
期数:
2017年05期
页码:
18-21
栏目:
试验研究
出版日期:
2017-10-31

文章信息/Info

Title:
Changes of carotenoids content in flower petal of Rhododendron molle during flower development
文章编号:
1001-7380（2017）05-0018-04
作者:
何丽斯; 肖政*; 苏家乐; 刘晓青; 李畅; 陈尚平
江苏省农业科学院休闲农业研究所/江苏省高效园艺作物遗传改良重点实验室，江苏 南京 210014
Author(s):
HE Li-si;  XIAO Zheng*;  SU Jia-le;  LIU Xiao-qing;  LI Chang;  CHEN Shang-ping
Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nangjing 210014, China
关键词:
羊踯躅; 类胡萝卜素; 高效液相色谱; 叶黄质; β-胡萝卜素
Keywords:
Rhododendron molle ;  Carotenoids;  High performance liquid chromatography;  Lutein;  β-Carotene
分类号:
Q945.6+4
DOI:
10.3969/j.issn.1001-7380.2017.05.004
文献标志码:
A
摘要:
为揭示羊踯躅的花瓣花色形成机理和花色新品种选育提供参考。以羊踯躅花发育的4个时期花瓣为材料，采用高效液相色谱法(HPLC法)测定4种类胡萝卜素的含量，并分析其变化规律。结果表明，高效液相色谱法可以对羊踯躅花瓣中的叶黄质、玉米黄质、α-胡萝卜素和β-胡萝卜素完全分离并进行分析。羊踯躅在花蕾期已经有大量类胡萝卜素积累。β-胡萝卜素在花发育过程中，其含量远高于α-胡萝卜素、叶黄质和玉米黄质，α-胡萝卜素的含量在整个花发育过程中含量均较低；β-胡萝卜素随着花的发育，其含量先降后升，在盛开期达到最高（95.25 μg/g FW），占检测类胡萝卜素总量的92.89%；叶黄质和玉米黄质则相反，先升后降，在花朵膨大期含量最高（48.15 μg/g FW和18.71 μg/g FW），分别占检测类胡萝卜素总量的42.18%和16.39%，在盛开期含量均较低。认为β-胡萝卜素是影响羊踯躅花瓣着色的一个主要因素，其含量变化与羊踯躅的花色形成密切相关。
Abstract:
The changes of carotenoids content in the flower petal of Rhododendron molle during flower development were investigated. Four kinds of carotenoids were separated and their contents were analyzed by high performance liquid chromatography (HPLC). The results showed that there were a large number of carotenoids accumulated in the petal during the flower early development. The content of β-carotene was much higher than the other carotenoids during the flower development while the content of α-carotene was always low. The content of β-carotene tended to decline at the flower expanding stage and then rise to 95-25 μg/g FW at the full flowering stage, accounting for 92.89% of the total carotenoid content. On the contrary, the content of lutein and zeaxanthin rose to 48.15 and 18.71 μg/g FW at the flower expanding stage, accounting for 42.18% and 16.39% of total carotenoid content, respectively whereas their contents were low at the full flowering stage. It was indicated that β-carotene played an important role in the coloring of R. molle flower, and the changes of β-carotene content was closely related with the flower color formation of R. molle.

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备注/Memo

备注/Memo:
收稿日期：2017-08-23;修回日期：2017-09-18
基金项目：国家自然科学基金“基于转录组学挖掘调控羊踯躅花瓣黄色性状形成的关键基因”(31600570)；江苏省自然科学基金“调控羊踯躅花色突变体花瓣呈色的关键基因挖掘”(BK20150548)；江苏省林业科技创新与推广项目“杜鹃花新品种‘蝶舞’、‘蝶恋’工厂化育苗技术及应用推广”（lykj[2017]48）
作者简介：何丽斯（1981- ），女，广东中山人，副研究员，博士。主要从事观赏植物生物工程及生理方面的研究。E-mail: alicehelisi@foxmail.com。
*通信作者：肖政（1984- ），男，福建三明人，副研究员，博士。主要从事花卉遗传育种研究。E-mail: xzforestry@163.com。
更新日期/Last Update: 2017-11-21