[1]黄益基,吕秀立*,付 瑞,等.金红茵芋离体快速繁殖技术的研究[J].江苏林业科技,2017,44(04):8-12.[doi:10.3969/j.issn.1001-7380.2017.04.002]
 HUANG Yi-ji,LYU Xiu-li,*,et al.Research of in vitro rapid propagation technique of Skimmia japoniac ‘rnbella’[J].Journal of Jiangsu Forestry Science &Technology,2017,44(04):8-12.[doi:10.3969/j.issn.1001-7380.2017.04.002]
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金红茵芋离体快速繁殖技术的研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第44卷
期数:
2017年04期
页码:
8-12
栏目:
试验研究
出版日期:
2017-08-31

文章信息/Info

Title:
Research of in vitro rapid propagation technique of Skimmia japoniac ‘rnbella’
文章编号:
1001-7380(2017)04-0008-05
作者:
黄益基12吕秀立134*付 瑞5郭小芳5方 腾6
1.上海市园林科学规划研究院,上海 200232;
2.浙江省长兴县水口乡林业工作站,浙江 长兴 313108;
3.上海城市困难立地绿化工程技术研究中心,上海 200232;
4.国家林木种质资源平台—上海子平台,上海 200232;
5.德州世纪风园艺科技创新有限公司,山东 德州 253500;
6.嘉善县笠歌生态科技有限公司,浙江 嘉善 314113
Author(s):
HUANG Yi-ji12 LYU Xiu-li134* FU Rui5 GUO Xiao-fang5 FANG Teng6
1.Shanghai Academy of Landscape Architecture Science and Planning, Shanghai 200232, China;
2.Forestry Station of Shuikou Town, Changxing County, Zhejiang, Changxing 313108, China;
3.Shanghai Engineering Research Center of Landscaping on Challenging Urban Sites, Shanghai 200232, China;
4. Shanghai Sub-platform,National Forest Genetic Resources Platform, Shanghai 200232, China;
5.Dezhou Shijifeng Horticulture Innovation CO., Ltd, Dezhou 253500, China;
6.Jiashan County Ecological Technology CO., Ltd, Zhejiang, Jiashan 3141113, China
关键词:
金红茵芋带腋芽茎段外植体诱导增殖生根基质
Keywords:
Skimmia japonica‘rnbella’ Nodal segment Explant Induction Multiplication Rooting Substrate
分类号:
Q 943.1;Q 949.752.7
DOI:
10.3969/j.issn.1001-7380.2017.04.002
文献标志码:
A
摘要:
为了建立完善的金红茵芋快速繁殖技术体系,以带腋芽茎段为外植体,对其进行了离体培养。结果表明:初代培养基以MS+0.2 mg/L ZT +0.04 mg/L IBA +5.0 mg/L GA3较为适宜,外植体诱导率最高达85%;最佳增殖培养基是MS+1.0 mg/L ZT +0.5 mg/L KT +0.1 mg/L IBA,培养30 d后,增殖率达3.6,试管苗高度达2.0 cm;生根培养基以1/2MS+1.0 mg/L IBA效果为最佳,生根率达90%;适时移栽入泥炭+蛭石+珍珠岩+有机肥(体积比为6∶2∶∶1)的混合基质中,成活率在80%以上。
Abstract:
The nodal segments with auxiliary bud from Skimmia japoniac ‘rnbella’ were used as explants for micro-propagation. After the explants were cultured on the MS media with 0.2 mg/L ZT, 0.04 mg/L IBA and 5.0 mg/L GA3for 30 days, the sprout induction percentage reached 85%. During the multiplication stage of 30 days, the sprouts cultured on the MS medium with 1.0 mg/L ZT, 0.5 mg/L KT and 0.1 mg/L IBA could get shoot multiplication of 3.6, and height of 2.0 cm. In the consecutive rooting step, isolated shoots placed in 1/2 MS media containing 1.0 mg/L IBA could get the rooting rate of 90%. The survival of plantlets was more than 80% for the appropriate time of transplant, with transplant substrate mixture(containing peat soil, vermiculite, perlite and manure) fixed as a volume ratio of 6

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-06-06;修回日期:2017-07-11
基金项目:上海市种业发展项目“特色蔬菜资源收集、特色良种筛选及标准化繁育”(沪农科种字(2015)第8号);上海市崇明区可持续发展科技创新行动计划“岩白菜种植技术集成与示范应用”(CK2017-50)
作者简介:黄益基(1973-),男,浙江长兴人,工程师,大学本科毕业。主要从事林木良种选育工作。E-mail:skh9592@126.com。
*通信作者:吕秀立(1980-),女,山东德州人,高级工程师,硕士。专业方向:林木遗传育种。E-mail:tkdyun@163.com。
更新日期/Last Update: 2017-11-21