[1]何月秋,黄艾,李波.紫娇花组织培养中愈伤组织的诱导及植株再生[J].江苏林业科技,2015,42(06):19-22.[doi:doi:10.3969/j.issn.1001-7380.2015.06.005]
 HE Yue-qiu,HUANG Ai,LI Bo.Callus induction and plant regeneration in Tulbaghia violacea in vitro culture[J].Journal of Jiangsu Forestry Science &Technology,2015,42(06):19-22.[doi:doi:10.3969/j.issn.1001-7380.2015.06.005]
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紫娇花组织培养中愈伤组织的诱导及植株再生()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第42卷
期数:
2015年06期
页码:
19-22
栏目:
试验研究
出版日期:
2015-12-30

文章信息/Info

Title:
Callus induction and plant regeneration in Tulbaghia violacea in vitro culture
文章编号:
1001-7380(2015)06-0019-04
作者:
何月秋12黄艾1李波2
1.宁波城市职业技术学院,浙江宁波315100;
2.江苏碧云天科技有限公司,江苏丹阳212300
Author(s):
HE Yue-qiu12HUANG Ai1LI Bo2
1.Ningbo College of Vocational Technology,Ningbo 315100,China;
2.Jiangsu Biyuntian Science & Technology Limited Company,Danyang 212300,China
关键词:
紫娇花种子下胚轴子叶愈伤组织诱导植株再生
Keywords:
Tulbaghia violacea Seed Hypocotyl Cotyledon Callus Induction Plant regeneration
分类号:
Q949.71+8.25
DOI:
doi:10.3969/j.issn.1001-7380.2015.06.005
文献标志码:
A
摘要:
紫娇花种子经灭菌处理后,于试管内培养基中萌发,取幼苗的下胚轴、子叶,进行愈伤组织的诱导、不定芽的分化及试管苗生根技术的研究。结果显示,紫娇花种子最佳灭菌方式为0.1%升汞浸泡10 min,污染率可控制在37.0%,萌芽率达到86.3%;愈伤组织最适诱导部位为下胚轴,愈伤组织诱导最佳培养基为MS+0.2 mg/L 6-BA+2.0 mg/L 2,4-D,诱导率达到83.7%;紫娇花不定芽分化的最适培养基为MS+1.0 mg/L 6-BA +0.2 mg/L NAA,不定芽分化率为48.0%;最适生根培养基为1/4 MS+0.1 mg/L NAA +0.5% AC,生根率可接近100%。
Abstract:
Taking the hypocotyl and cotyledon excised from the aseptic plantlets of Tulbaghia violacea as explants, a plant regeneration protocol was developed about callus induction, adventitious bud differentiation, root formation. The results showed that the optimal sterilization method was 0.1% HgCl2 treatment for 10 min, with 37.0% pollution rate and 86.3% germination rate. For hypocotyl as optimal explant, the optimal callus inducing medium was MS with 2.0 mg/L 2,4-D+0.2 mg/L 6-BA, with 83.7% induction rate.Adventitious buds were effectively generated on MS with 1.0 mg/L 6-BA+0.2 mg/L NAA, with 48.0% differentiation rate.Roots were formed from the shoots on 1/4 MS supplemented with 0.1 mg/L NAA after 20 d, with the rooting rate almost reaching 100%.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-08-14;修回日期:2015-08-28
基金项目:浙江省宁波科技局双创项目(2012C91027);江苏省丹阳市高层次创新人才引进项目
作者简介:何月秋(1975- ),女,四川大邑人,副教授,研究方向:植物组织培养。E-mail:heyueqiu@nbcc.cn。
更新日期/Last Update: 2016-01-29