[1]陈少卿,宗树斌*,任焕焕.植物生长调节剂对四倍体甜叶菊扦插生根的影响[J].江苏林业科技,2024,51(06):14-21.[doi:10.3969/j.issn.1001-7380.2024.06.003]
 Chen Shaoqing,Zong Shubin*,Ren Huanhuan.The effect of different hormone treatments on therooting of tetraploid Stevia rebaudiana Bertoni cuttings[J].Journal of Jiangsu Forestry Science &Technology,2024,51(06):14-21.[doi:10.3969/j.issn.1001-7380.2024.06.003]
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植物生长调节剂对四倍体甜叶菊扦插生根的影响()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第51卷
期数:
2024年06期
页码:
14-21
栏目:
试验研究
出版日期:
2024-12-31

文章信息/Info

Title:
The effect of different hormone treatments on therooting of tetraploid Stevia rebaudiana Bertoni cuttings
文章编号:
1001-7380(2024)06-0014-08
作者:
陈少卿宗树斌*任焕焕
江苏农林职业技术学院,江苏 句容 212400
Author(s):
Chen ShaoqingZong Shubin*Ren Huanhuan
Jiangsu Vocational College of Agriculture and Forestry,Jurong 212400,China
关键词:
甜叶菊四倍体扦插植物生长调节剂生根率
Keywords:
Stevia rebaudiana BertoniTetraploidCuttingHormoneRooting rate
分类号:
Q945.52;S482.8;S567.23+9
DOI:
10.3969/j.issn.1001-7380.2024.06.003
文献标志码:
A
摘要:
四倍体甜叶菊相比二倍体植株具有营养器官巨大、活性成分含量高、抗逆性强等显著特征。扦插是四倍体植株繁殖的主要途径,该试验研究用不同植物生长调节剂处理对四倍体甜叶菊扦插生根的影响。分别用NAA,ABT和GA33种植物生长调节剂对四倍体甜叶菊“守田3号”插穗进行处理,采用双因素(不同质量浓度和不同处理时间)完全重复试验,对试验结果进行方差分析、多重比较。结果表明:综合生根率、生根数、根长3个指标分析,利用NAA最佳的处理为NAA质量浓度600 mg/L+浸泡20 min,利用ABT处理促进生根的最佳组合为ABT质量浓度1 000 mg/L+浸泡25 min,利用GA3处理最佳组合为GA3质量浓度100 mg/L+浸泡 10 min。
Abstract:
Compared with diploid plants, tetraploid Stevia rebaudiana has significant characteristics such as huge nutritional organs, high content of active ingredients, and strong stress resistance. Cutting propagation is the main way for tetraploid plant propagation. Studying the effects of different hormone treatments on the rooting of tetraploid stevia cuttings is of great significance for accelerating the rapid propagation. In this experiment, three hormones, NAA, ABT, and GA3, were used to treat the rooting of tetraploid S. rebaudiana ‘Shoutian 3’ cuttings. Different concentration and treatment time factors were designed in the fully repeated experiment. The experimental results showed that the optimal treatment for comprehensive rooting rate, rooting number, and root length using NAA was 600 mg/L followed by soaking for 20 minutes. The optimal combination for promoting rooting using ABT treatment was 1 000 mg/L followed by soaking for 25 minutes. The optimal GA3 treatment was 100 mg/L followed by soaking for 10 minutes.

参考文献/References:

[1]SINGHM,SAHARAN VDAYMA J,etal.In vitro propagation of Stevia rebaudiana (Bertoni): an overview[J].International Journal of Current Microbiology and Applied Sciences,2017,6(7): 1010-1022.

[2]李红,杨岚,向增旭.甜叶菊同源四倍体离体诱导及鉴定[J].西北植物学报,2012,32(8):1692-1697.
[3]TADHANI M B,PATEL V H,SUBHASH R.In vitro antioxidant activities of Stevia rebaudiana leaves and callus[J].Journal of Food Composition and Analysis,2007,20(3): 323-329.
[4]CARRERA-LANESTOSA A,MOGUEL-ORDONEZ Y,et al.Steviarebaudiana Bertoni: a natural alternative for treating diseases associated with metabolic syndrom[J].Journal of Medicinal Food,2017,20(10): 933-943.
[5]JEEVAN J,KAURM,MISHRA V,et al.Sweet future of stevia: a magical sweetener[J].Asian Journal of Pharmaceutical and Clinical Research,2018,11(2): 36-42.
[6]ARUMUGAM B,SUBRAMANIAM A,ALAGAR A J P.Stevia as a natural sweetener: a review[J].Cardiovascular & Hematological agents in Medicinal Chemistry,2020,18(2): 94-103.
[7]JANS A,HABIB N,SHINWARI Z K,et al.The anti-diabetic activities of natural sweetener plant rstevia: an updated review[J].SN Applied Sciences,2021,3: 517.
[8]KARIMI M,AHMADI A,HASHEMI J,et al.Plant growth retardants (PGRs) affect growth and sarymetabolit biosynthesis in Stevia rebaudiana Bertoni under drought stress[J].South African Journal of Botany,2019,121: 394-401.
[9]WOLWER-RIECK U.The leaves of Stevia rebaudiana (Bertoni),their constituents and the analyses there of: a review[J].Journal of Agricultural and Food Chemistry,2012,60(4): 886-895.
[10]GASMALLA M A A, YANG R J,HUA X.Stevia rebaudiana Bertoni: an alternative sugar replacer and its application in food industry[J].Food Engineering Reviews,2014,6(4): 150-162.
[11]SAMUEL P,AYOOB K T,MAGNUSON B A,et al.Stevia leaf to stevia sweetener: exploring its science benefits and future potential[J].The Journal of Nutrition,2018,148(7): 1186-1205.
[12]SALEHI B,LOPEZ M D,MARTINEZ-LOPE Z,et al.Stevia rebaudiana Bertoni bioactive effects: from in vivo to clinical trials towards future therapeutic approaches[J].Phytotherapy Research, 2019,33(11): 2904-2917.
[13]SIMLAT M,PTAK A,WOJTOWIC Z,et al.The content of phenolic compounds in Stevia rebaudiana (Bertoni) plants derived from melatonin and NaCl treated seeds[J].Plants,2023,12(4): 780.
[14]陈绍潘,陈绍裘,杨英华,等.秋水仙碱诱变甜菊多倍体的研究[J].武汉植物学研究,1995,13(1):1-7.
[15]李雅婷,王红娟,向增旭.甜叶菊二倍体与同源四倍体生理特征及AFLP分析[J].核农学报,2015,29(11):2103-2109.
[16]李雅婷,王红娟,向增旭.甜叶菊同源四倍体与二倍体基因组差异分析[J].中国农学通报,2015,31(28):112-116.
[17]杨岚,李红,向增旭.二倍体和同源四倍体甜叶菊的DNA甲基化差异分析[J].核农学报,2013,27(8):1125-1130.
[18]邱国金,韩广发,孙其松,等.不同基质与生长素对紫薇新品种仑山1号嫩枝扦插育苗的影响[J].贵州农业科学,2019,47(9):66-68.
[19]狄清琳.七种素馨属植物扦插育苗及其离体培养研究[D].南宁:广西大学,2019.
[20]张阳锋,任征,林明平,等.不同激素种类及浓度对红厚壳嫩枝扦插育苗的影响[J].热带作物学报,2020,41(11):2183-2189.
[21]刘林,孔青,杜明芸.赤霉素在三色堇扦插繁殖上的应用[J].山东林业科技,2005(5):24-25.
[22]董胜军,刘明国,戴菲.山杏嫩枝扦插生根过程中插穗内源激素含量的变化[J].经济林研究,2013,31(4):108-114.
[23]李燕,孙玲,方明慧,等.赤霉素处理对甜叶菊扦插苗生根的影响初探[J].南方农业,2019(5):117-119.

备注/Memo

备注/Memo:
收稿日期:2024-08-01;修回日期:2024-09-04
基金项目:江苏现代农业(花卉)产业技术体系“句容推广示范基建设”项目[JATS2022(382)]
作者简介:陈少卿(1982- )男,江苏兴化人,农艺师,硕士。主要从事园林植物栽培,药用植物繁育技术研发。
*通信作者:宗树斌(1982-)男,山东巨野人,教授,硕士。主要从事园林植物工厂化繁育技术研发及应用。
更新日期/Last Update: 2025-01-23