[1]瞿印权,陈思凯,宋鹍鹏,等.绿竹等30个竹种ISSR遗传多样性分析及指纹图谱构建[J].江苏林业科技,2017,44(01):1-6.[doi:10.3969/j.issn.1001-7380.2017.01.001]
　QU Yin-quan,CHEN Si-kai,SONG Kun-peng,et al.Genetic diversity and DNA fingerprint of 30 bamboo species using ISSR markers[J].Journal of Jiangsu Forestry Science &Technology,2017,44(01):1-6.[doi:10.3969/j.issn.1001-7380.2017.01.001]
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绿竹等30个竹种ISSR遗传多样性分析及指纹图谱构建()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第44卷
期数:
2017年01期
页码:
1-6
栏目:
试验研究
出版日期:
2017-03-05

文章信息/Info

Title:
Genetic diversity and DNA fingerprint of 30 bamboo species using ISSR markers
文章编号:
1001-7380（2017）01-0001-06
作者:
瞿印权1; 陈思凯1; 宋鹍鹏1; 刘家琪1; 杨德明1; 李炎梅2; 何天友2; 荣俊冬1; 陈礼光1; 郑郁善1; 2*
1.福建农林大学林学院，福建福州350002；
2.福建农林大学艺术学院园林学院，福建福州350002
Author(s):
QU Yin-quan1;  CHEN Si-kai1;  SONG Kun-peng1;  LIU Jia-qi1;  YANG De-ming1;  LI Yan-mei2;  HE Tian-you2;  RONG Jun-dong1;  CHEN Li-guang1;  ZHENG Yu-shan1; 2*
1. College of forestry，Fujian Agriculture and Forestry University，Fuzhou 350002, China;
2. College of Art & Landscape Architecture, Fujian Agriculture and Forestry University，Fuzhou 350002, China
关键词:
竹种; 遗传多样性; ISSR聚类分析; DNA指纹图谱
Keywords:
Bamboo species;  Genetic diversity;  ISSR cluster analysis;  DNA fingerprint
分类号:
S795；Q523+.8
DOI:
10.3969/j.issn.1001-7380.2017.01.001
文献标志码:
A
摘要:
为了研究30个竹种的遗传多样性和亲缘关系，建立其DNA指纹图谱，为竹亚科部分植物的分类及种质鉴定提供参考，利用优化的ISSR-PCR体系，筛选出18个多态性较好的的引物，对30个竹种的DNA进行PCR扩增。结果显示，18条引物扩增出的多态性位点占100%,平均每个引物扩增出14个位点,且DNA片段长度在200—3 000 bp之间;30个竹种间的平均遗传距离为0.441 4，平均遗传相似度为0.644 44; 在遗传距离0.51处进行ISSR聚类分析，可以将竹种分成6组，这与传统的分类大多比较吻合。说明30个竹种具有相当丰富的遗传多样性和基因库,各竹种间有一定的遗传差异和亲缘关系; ISSR技术分析竹种间亲缘关系和遗传多样性具有较高的精确性，可作为竹种分类的参考技术。
Abstract:
In order to study the genetic diversity of 30 bamboo species and establish the DNA fingerprints and provide some identification of bamboos, the optimized ISSR-PCR system was utilized to screen out 18 primers with good polymorphism and their DNAs were used for the PCR amplification. Results showed that 18 screened primers produced 253 loci, which were polymorphisms, and the percentage of polymorphic loci was up to 100%.The average value of the effective number of alleles was 1.575 6,the average value of the Nei’s genetic diversity was 0.343 7 and the Shannon’s index was 0.517 8.The genetic distance of 30 bamboo species ranged from 0.104 to 0.620 7.Based on cluster tree and the genetic distance,the 30 bamboo species were divided into two groups or six small groups. In addition,the DNA fingerprints for the bamboo species were established by 8 polymorphic ISSR primers. It was concluded that there existed more accurate information on genetic relationship,diversity among 30 bamboo species, suggesting ISSR molecular markers could be effectively utilized in genetic diversity and establishment of fingerprints of these 30 bamboo species.

参考文献/References:

[1]郑林. 酸竹属遗传多样性研究[D]. 福州：福建农林大学, 2011.
[2]肖永太. 大明竹属指纹图谱的研究[D]. 福州：福建农林大学, 2011.
[3]DESAI P, GAJERA B, MANKAD M, et al. Comparative assessment of genetic diversity among Indian bamboo genotypes using RAPD and ISSR markers [J]. Molecular Biology Reports, 2015, 42(8):1265-1273.
[4]ZHU S, LIU T, TANG Q, et al. Evaluation of bamboo genetic diversity using morphological and SRAP analyses [J]. Russian Journal of Genetics, 2014, 50(3):306-313.
[5]郭振华, 李德铢. 竹亚科系统学和生物地理学研究进展及存在的问题[J]. 云南植物研究, 2002, 24(4):431-438,462.
[6]朱岩芳, 祝水金, 李永平, 等. ISSR分子标记技术在植物种质资源研究中的应用[J]. 种子, 2010, 29(2):55-59.
[7]FAROOQ S, AZAM F. Molecular Markers in Plant Breeding-I: Concepts and Characterization[J]. Pakistan Journal of Biological Sciences, 2002, 5(10):1135-1140.
[8]LANDJEVA S, KORZUN V, BOMER A. Molecular markers: actual and potential contributions to wheat genome characterization and breeding[J]. Euphytica, 2007, 156(3):271-296.
[9]WENG M, LIU B, JIN D, et al. Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers[J]. Journal of Applied Phycology, 2005, 17(1):91-97.
[10]WANG L X, LI H B, GU T C, et al. Assessment of wheat variety stability using SSR markers[J]. Euphytica, 2014, 195(3):435-452.
[11]mKASTURE A, KRISHNAMURTHY R, RAJKUMAR K. Genetic variation in the endangered Indian sweet flag ( Acorus calamus L.) estimated using ISSR and RAPD markers[J]. Journal of Applied Research on Medicinal and Aromatic Plants, 2016,3(3):112-119.
[12]林 立, 王志龙, 付 涛, 等. 39个樱花品种亲缘关系的ISSR分析[J]. 植物研究, 2016,36(2):297-304.
[13]黄树军, 陈礼光, 肖永太,等. 大明竹属遗传多样性ISSR分析及DNA指纹图谱研究[J]. 生态学报, 2013, 33(24):7863-7871.
[14]娄永峰, 杨海芸, 张有珍, 等. 部分竹类植物遗传变异的AFLP, ISSR和SRAP分析[J]. 福建林学院学报, 2011, 31(1):38-43.
[15]娄永峰. 雷竹不同变异类型的遗传多样性分析[D]. 杭州：浙江农林大学, 2010.
[16]张志欣. 簕竹属部分竹种间亲缘关系的RAPD标记研究[D]. 福州：福建农林大学, 2008.
[17]李喜凤, 邱天宝, 张红梅, 等. 蒲公英ISSR-PCR反应体系及扩增程序的建立与优化[J]. 中国实验方剂学杂志, 2012, 18(16):119-122.
[18]刘晓伶, 郝建平, 付 琳. 山西野生黄芩种质资源的RAPD分析[J]. 山西农业科学, 2016, 44(3):204-206.
[19]杨妙贤, 潘伟明, 周玲艳, 等. 3个野生毛花猕猴桃居群的SSR多样性分析[J]. 华南师范大学学报(自然科学版), 2014, 46(4):87-92.
[20]KAWAMURA K, KAWANABE T, SHIMIZU M, et al. Genetic distance of inbred lines of Chinese cabbage and its relationship to heterosis [J]. Plant Gene, 2016, 5:1-7.
[21]缪恒彬,陈发棣,赵宏波, 等. 应用ISSR对25个小菊品种进行遗传多样性分析及指纹图谱构建[J]. 中国农业科学,2008,41(11):3735-3740.
[22]魏玉杰,张金文,何庆祥, 等. 不同生态区罂粟种质的遗传多样性ISSR分析[J]. 植物遗传资源学报,2012,13(2):239-243.
[23]曾建飞.中国植物志(第九卷第一分册)[M] .北京：科学出版社，1996：1-781.

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备注/Memo

备注/Memo:
收稿日期：2016-09-28;修回日期：2016-10-12基金项目：福建省农业科技重大专项“特色林木种质材料选育与高效培育关键技术研究”(2013NZ0001) ; 福建省科技重大项目“中小型竹资源优良品种选择和定向培育关键技术研究”作者简介：瞿印权(1992- )，男，江苏南通人，硕士研究生。主要从事森林培育理论与技术研究。E-mail:386142881@qq.com。*通信作者：郑郁善(1960- )，男，福建福州人，教授，博士。主要从事竹林培育研究，E-mail: zys1960@163.com。
更新日期/Last Update: 2017-03-03