[1]郑纪伟,孙冲,周洁,等.基于EST-SSR标记的欧洲红皮柳遗传变异分析[J].江苏林业科技,2016,43(06):6-11.[doi:10.3969/j.issn.1001-7380.2016.06.002]
　ZHENG Ji-wei,SUN Chong,ZHOU Jie,et al.Analysis of genetic variation of Salix purpurea based on EST-SSR markers[J].Journal of Jiangsu Forestry Science &Technology,2016,43(06):6-11.[doi:10.3969/j.issn.1001-7380.2016.06.002]
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基于EST-SSR标记的欧洲红皮柳遗传变异分析()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第43卷
期数:
2016年06期
页码:
6-11
栏目:
试验研究
出版日期:
2016-12-30

文章信息/Info

Title:
Analysis of genetic variation of Salix purpurea based on EST-SSR markers
文章编号:
1001-7380（2016）06-0006-06
作者:
郑纪伟1; 孙冲1; 2; 周洁1; 何旭东1*
1. 江苏省林业科学研究院，江苏南京211153；
2. 南京林业大学，江苏南京210037
Author(s):
ZHENG Ji-wei1;  SUN Chong1; 2;  ZHOU Jie1;  HE Xu-dong1*
1. Jiangsu Academy of Forestry, Nanjing 211153, China;
2. Nanjing Forestry University, Nanjing 210037, China
关键词:
欧洲红皮柳; 微卫星; 遗传变异; 亲缘关系
Keywords:
S. purpurea; SSR; Genetic variation; Genetic relationship
分类号:
S792.12;Q754
DOI:
10.3969/j.issn.1001-7380.2016.06.002
文献标志码:
A
摘要:
利用25个柳树EST-SSR标记对欧洲红皮柳18个个体进行基因分型，并通过标记多态性来检验个体间的遗传变异情况。结果表明：25个标记共检测到100条等位片段，每个位点等位基因数从1—12个不等，平均4个。其中23个标记表现为多态性，其观测杂合度(Ho)变化范围为0.055 5到1.0，平均为0.627 2；期望杂合度(He)变化范围为0.055 5到0.852 3，平均为0.567 8；多态信息量(PIC)变化范围为0.812 5到0.052 5，平均值为0.494 1。聚类分析显示，在遗传相似度为0.52处，欧洲红皮柳18个个体分为2组。该研究为欧洲红皮柳核心种质的收集与利用提供了理论依据。
Abstract:
In this paper, 18 individuals of Salix purpurea were genotyped by 25 EST-SSR markers，and the genetic variations of these individuals were evaluated through marker polymorphism. The result showed that 100 alleles had been detected from 25 EST-SSR in total, of which 23 markers were polymorphic. The number of alleles ranged from 1 to 12, with an average of 4 alleles per locus. The observed heterozygosity (Ho), the expected heterozygosity (He) and the polymorphic information content (PIC) varied with locus from 0.055 5 to 1.0 (mean equals 0.627 2), 0.055 5 to 0.852 3 (mean equals 0.567 8) and 0.052 5 to 0.812 5 (mean equals 0.494 1) respectively. Two main groups were divided with the similarity coefficient of 0.52 by dendrogram analysis. This study could provide a theoretical basis for the collection and utility of S. purpurea core germplasm.

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备注/Memo

备注/Memo:
收稿日期：2016-11-25;修回日期：2016-12-03
基金项目：国家林业局948项目“高生物量灌木柳遗传资源及培育技术引进”(2012-4-38)；江苏省农业科技自主创新资金项目“观赏柳树新品种选育”[CX(14)2024]
作者简介：郑纪伟(1986- )，男，河南嵩县人，硕士。主要从事植物学研究。
*通信作者：何旭东(1981- )，男，江苏句容人，副研究员，博士。主要从事林木遗传育种研究。E-mail：hxd_519@163.com。
更新日期/Last Update: 2016-12-30