[1]亓希武,林沂,陈泽群,等.金银花花发育过程中内源激素含量变化规律研究[J].江苏林业科技,2022,49(06):1-6.[doi:10.3969/j.issn.1001-7380.2022.06.001]
　Qi Xiwu,Lin Yi,Chen Zequn,et al.Study on the changing of three endogenous hormones during the development of Lonicera japonica flowers[J].Journal of Jiangsu Forestry Science &Technology,2022,49(06):1-6.[doi:10.3969/j.issn.1001-7380.2022.06.001]
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金银花花发育过程中内源激素含量变化规律研究()
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《江苏林业科技》[ISSN:1001-7380/CN:32-1236/S]

卷:
第49卷
期数:
2022年06期
页码:
1-6
栏目:
试验研究
出版日期:
2022-12-31

文章信息/Info

Title:
Study on the changing of three endogenous hormones during the development of Lonicera japonica flowers
文章编号:
1001-7380（2022）06-0001-06
作者:
亓希武1; 林沂1; 陈泽群1; 房海灵1; 仇丽云2; 梁呈元1*
1．江苏省中国科学院植物研究所，江苏 南京 210014；
2．常州金坛冰蕊金银花专业合作社，江苏 常州 213200
Author(s):
Qi Xiwu1;  Lin Yi1;  Chen Zequn1;  Fang Hailing1;  Qiu Liyun2;  Liang Chengyuan1*
1.Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China;
2.Changzhou Jintan Bingrui Honeysuckle Professional Cooperative，Changzhou 213200, China
关键词:
金银花; 花发育; IAA; ABA; GA3; 变化规律
Keywords:
Lonicera japonica; Flower development; IAA; ABA; GA3; Changing rule
分类号:
Q945.6+4；Q946.885；S567.7+9
DOI:
10.3969/j.issn.1001-7380.2022.06.001
文献标志码:
A
摘要:
金银花是一种重要的大宗中药材，具有多种生物学活性，临床应用广泛。金银花主要以其干燥花蕾入药，花蕾产量和品质与药材质量和经济效益密切相关。该研究利用高效液相色谱-串联质谱（HPLC-MS/MS）方法检测了金银花花发育过程中3种内源激素IAA、ABA和GA3的含量变化规律。结果表明3种内源激素的变化趋势并不相同，IAA质量浓度呈现出先升高后降低并持平的变化趋势，其含量的持续增加促进了花的发育；ABA和GA3的质量浓度在白蕾期到金花期的过程中迅速上升，说明高质量浓度的ABA和GA3促进开花过程。3种激素的平衡在不同发育阶段呈现出不同变化趋势，IAA/ABA比值的升高有利于花蕾初期的发育，而其比值的下降则促进花蕾后期的发育和开花过程；IAA/GA3和ABA/GA3比值的升高促进花蕾的发育和开花，而其比值的下降可能促进了花色由白转黄和衰老过程。该研究对揭示植物内源激素调控金银花花发育的机制提供了重要的基础数据。
Abstract:
Lonicera japonica Thunb. is an important medicinal plant with a variety of biological activities and is widely used in clinical medicine. L. japonica is mainly used as medicine with its dried flower buds, and the yield and quality of flower buds are closely related to the quality of medicinal materials and economic benefits. In this study, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to detect the changes in the content of three endogenous hormones IAA, ABA and GA3 during the development of L. japonica flowers. The results showed that the changing trends of the three endogenous hormones were not the same. The concentration of IAA showed a trend of increasing at first, then decreasing and remaining flat, and the continuous increase of its content promoted flower development. The concentrations of ABA and GA3 increased rapidly from the white bud stage to the golden flowering stage, indicating that high concentrations of ABA and GA3 promoted the flowering process. The balance of the three hormones showed different trends at different developmental stages. An increase in the IAA/ABA ratio favored the development of buds at the early stage, while a decrease in its ratio promoted the development of buds at the later stage and the flowering process; an increase in the IAA/GA3 and ABA/GA3 ratios promoted the development of buds and flowering, while a decrease in their ratios may have promoted the process of flower color changing from white to yellow and senescence. This study provides important basic data for revealing the mechanism of plant endogenous hormones regulating L. japonica flower development.

参考文献/References:

[1]中华人民共和国国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2020.
[2]SHANG X,PAN H,LI M,et al.Lonicera japonica Thunb: Ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine [J]. Journal of Ethnopharmacology,2011,138(1):1-21.
[3]KU S K,SEO B I,PARK J H,et al.Effect ofLonicerae Flos extracts on reflux esophagitis with antioxidant activity[J]. World Journal of Gastroenterology（WJG）,2009,15(38): 4799.
[4]BOUCH F, LOBET G,TOCQUIN P,et al.FLOR-ID: an interactive database of flowering-time gene networks in Arabidopsis thaliana [J]. Nucleic Acids Research,2016,44(D1):D1167-D1171.
[5]CAMPOS-RIVERO G,OSORIO-MONTALVO P,S NCHEZ-BORGES R,et al.Plant hormone signaling in flowering: An epigenetic point of view [J]. Journal of Plant Physiology,2017,214: 16-27.
[6]黎家,李传友.新中国成立70年来植物激素研究进展[J]. 中国科学:生命科学,2019,49(10):1227-1281.
[7]MARTIGNAGO D,SIEMIATKOWSKA B,LOMBARDI A,et al.Abscisic acid and flowering regulation: many targets, different places[J]. International Journal of Molecular Sciences,2020,21(24):9700.
[8]BAO S,HUA C,SHEN L,et al.New insights into gibberellin signaling in regulating flowering in Arabidopsis[J]. Journal of Integrative Plant Biology,2020,62(1):118-131.
[9]SHANG M,WANG X,ZHANG J,et al.Geneticregulation of GA metabolism during vernalization, floral bud initiation and development in Pak choi (Brassica rapa ssp. chinensis Makino)[J]. Frontiers in Plant Science,2017,8:1533.
[10]LIU X,LYU S,LIU R,et al.Transcriptomic analysis reveals the roles of gibberellin-regulated genes and transcription factors in regulating bolting in lettuce (Lactuca sativa L.)[J]. PLoS One,2018,13(2):e0191518.
[11]CHANG M Z,HUANG C H.Effects of GA3 on promotion of flowering in Kalanchoe spp[J]. Scientia Horticulturae,2018,238:7-13.
[12]文静,孔维军,罗红梅,等.植物内源激素检测方法新进展[J]. 中南药学,2014,12(1):47-52.
[13]王艳红,檀华蓉,高丽萍,等.高效毛细管电泳分离多种植物激素的方法研究[J]. 激光生物学报,2008,17(6):840-844,852.
[14]李玉璇,段春凤,关亚风.植物样品中内源性植物激素时空分布的研究进展[J]. 色谱,2019,37(8):806-814.
[15]LI J,YE C,CHANG C.Comparative transcriptomics analysis revealing flower trichome development during flower development in twoLonicera japonica Thunb. cultivars using RNA-seq [J]. BMC Plant Biology,2020,20:341.
[16]李天雪,胡玉涛,韦笑,等.超高效液相色谱-串联质谱同时测定盐碱胁迫金银花中3种内源激素[J]. 江苏农业科学,2019,47(11):229-233.
[17]牛辉陵,张洪武,边媛,等.枣花分化发育过程及其内源激素动态研究[J]. 园艺学报,2015,42(4):655-664.
[18]杨贵先,杨晓霞,王洋洋,等.南瓜花发育过程中内源激素和多胺含量变化研究[J].中国科技论文,2016,11(18):2096-2099.
[19]WANG T,YANG B,GUAN Q, et al.Transcriptional regulation of Lonicera japonica Thunb. during flower development as revealed by comprehensive analysis of transcription factors [J]. BMC Plant Biology,2019,19:198.
[20]王英,赵杨,冉洁.棕榈雌雄花发育过程中内源激素的变化[J]. 种子,2018,37(3):7-11.
[21]FU X D,HARBERD N P.Auxin promotes Arabidopsis root growth by modulating gibberellin response[J]. Nature,2003,421(6924): 740-743.

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备注/Memo

备注/Memo:
收稿日期：2022-07-22;修回日期：2022-08-27
基金项目：江苏省林业科技创新与推广项目“金银花林下间种模式研究与示范”(LYKJ[2021]20)；常州市科技支撑计划项目“植物生长调节剂调控金银花花期的技术研究与示范”(CE20202006)
作者简介：亓希武（1986- ），男，山东临沂人，副研究员，博士。主要从事药用植物资源研究。
*通信作者：梁呈元（1972- ），男，江西吉安人，研究员，博士。主要从事药用植物资源研究。E-mail：liangcy618@cnbgnet
更新日期/Last Update: 2023-04-04